System Maintenance Notice

Please note that maintenance work is scheduled between 4 AM and 5 AM - CET. During this time, you may experience reduced performance. Thanks for your patience.

Publications

Journal Article Epublish 16 citations

PubMed: 25502228 DOI: 10.1371/journal.pone.0114412

Blood thixotropy in patients with sickle cell anaemia: role of haematocrit and red blood cell rheological properties.

PLoS One 9(12):e114412

Authors: Jens Vent-Schmidt, Xavier Waltz, Marc Romana, Marie-Dominique Hardy-Dessources, Nathalie Lemonne, Marie Billaud, Maryse Etienne-Julan, Philippe Connes

Affiliations

  • Inserm UMR 1134, Hôpital Ricou, CHU de Pointe-à-Pitre, 97157, Pointe-à-Pitre, Guadeloupe, France.
  • Inserm UMR 1134, Hôpital Ricou, CHU de Pointe-à-Pitre, 97157, Pointe-à-Pitre, Guadeloupe, France; Laboratory of Excellence GR-Ex « The red cell: from genesis to death », PRES Sorbonne Paris Cité, 75015, Paris, France; Laboratory ACTES (EA 3596), Department of Physiology, French West Indies and Guiana University, Pointe-à-Pitre, Guadeloupe, France.
  • Inserm UMR 1134, Hôpital Ricou, CHU de Pointe-à-Pitre, 97157, Pointe-à-Pitre, Guadeloupe, France; Laboratory of Excellence GR-Ex « The red cell: from genesis to death », PRES Sorbonne Paris Cité, 75015, Paris, France.
  • Unité Transversale de la Drépanocytose, CHU de Pointe-à-Pitre, 97157, Pointe-à-Pitre, Guadeloupe, France.
  • Inserm UMR 1134, Hôpital Ricou, CHU de Pointe-à-Pitre, 97157, Pointe-à-Pitre, Guadeloupe, France; Laboratory of Excellence GR-Ex « The red cell: from genesis to death », PRES Sorbonne Paris Cité, 75015, Paris, France; Unité Transversale de la Drépanocytose, CHU de Pointe-à-Pitre, 97157, Pointe-à-Pitre, Guadeloupe, France.
  • Inserm UMR 1134, Hôpital Ricou, CHU de Pointe-à-Pitre, 97157, Pointe-à-Pitre, Guadeloupe, France; Laboratory of Excellence GR-Ex « The red cell: from genesis to death », PRES Sorbonne Paris Cité, 75015, Paris, France; Institut Universitaire de France, Paris, France; Laboratoire CRIS EA647, Université Claude Bernard Lyon 1, 69100, Villeurbanne, France.

Abstract

We compared the blood thixotropic/shear-thinning properties and the red blood cells' (RBC) rheological properties between a group of patients with sickle cell anaemia (SS) and healthy individuals (AA). Blood thixotropy was determined by measuring blood viscosity with a capillary viscometer using a "loop" protocol: the shear rate started at 1 s-1 and increased progressively to 922 s-1 and then re-decreased to the initial shear rate. Measurements were performed at native haematocrit for the two groups and at 25% and 40% haematocrit for the AA and SS individuals, respectively. RBC deformability was determined by ektacytometry and RBC aggregation properties by laser backscatter versus time. AA at native haematocrit had higher blood thixotropic index than SS at native haematocrit and AA at 25% haematocrit. At 40% haematocrit, SS had higher blood thixotropic index than AA. While RBC deformability and aggregation were lower in SS than in AA, the strength of RBC aggregates was higher in the former population. Our results showed that 1) anaemia is the main modulator of blood thixtropy and 2) the low RBC deformability and high RBC aggregates strength cause higher blood thixotropy in SS patients than in AA individuals at 40% haematocrit, which could impact blood flow in certain vascular compartments.

MeSH

Erythrocytes, Anemia, Blood Cells

Cited by