System Maintenance Notice

Please note that maintenance work is scheduled between 4 AM and 5 AM - CET. During this time, you may experience reduced performance. Thanks for your patience.

Publications

Journal Article Research Support, Non-U.S. Gov't Epublish 25 citations

PubMed: 33091018 DOI: 10.1371/journal.pone.0240307

DNA damage and health effects in juvenile haddock (Melanogrammus aeglefinus) exposed to PAHs associated with oil-polluted sediment or produced water.

PLoS One 15(10):e0240307

Authors: Sonnich Meier, Ørjan Karlsen, Jeremie Le Goff, Lisbet Sørensen, Elin Sørhus, Daniela M Pampanin, Carey E Donald, Per Gunnar Fjelldal, Evgenia Dunaevskaya, Marta Romano, Ilaria Caliani, Silvia Casini, André S Bogevik, Pål A Olsvik, Mark Myers, Bjørn Einar Grøsvik

Affiliations

  • Institute of Marine Research, Bergen, Norway.
  • ADn'tox, Bâtiment Recherche, Centre François Baclesse, Caen, France.
  • SINTEF Ocean AS, Environment and New Resources, Trondheim, Norway.
  • Department of Chemistry Bioscience and Environmental Engineering, Faculty of Science and Technology, University of Stavanger, Stavanger, Norway.
  • NORCE, Randaberg, Norway.
  • Department of Physical, Earth and Environmental Sciences, University of Siena, Siena, Italy.
  • Nofima AS - Norwegian Institute of Food, Fisheries Aquaculture Research, Fyllingsdalen, Norway.
  • Nord Univ, Fac Biosci & Aquaculture, Bodo, Norway.
  • Myers Ecotoxicology Services, LLC, Shoreline, Washington, United States of America.

Abstract

The research objective was to study the presence of DNA damages in haddock exposed to petrogenic or pyrogenic polyaromatic hydrocarbons (PAHs) from different sources: 1) extracts of oil produced water (PW), dominated by 2-ring PAHs; 2) distillation fractions of crude oil (representing oil-based drilling mud), dominated by 3-ring PAHs; 3) heavy pyrogenic PAHs, mixture of 4/5/6-ring PAHs. The biological effect of the different PAH sources was studied by feeding juvenile haddock with low doses of PAHs (0.3-0.7 mg PAH/kg fish/day) for two months, followed by a two-months recovery. In addition to the oral exposure, a group of fish was exposed to 12 single compounds of PAHs (4/5/6-ring) via intraperitoneal injection. The main endpoint was the analysis of hepatic and intestinal DNA adducts. In addition, PAH burden in liver, bile metabolites, gene and protein expression of CYP1A, GST activity, lipid peroxidation, skeletal deformities and histopathology of livers were evaluated. Juvenile haddock responded quickly to both intraperitoneal injection and oral exposure of 4/5/6-ring PAHs. High levels of DNA adducts were detected in livers three days after the dose of the single compound exposure. Fish had also high levels of DNA adducts in liver after being fed with extracts dominated by 2-ring PAHs (a PW exposure scenario) and 3-ring PAHs (simulating an oil exposure scenario). Elevated levels of DNA adducts were observed in the liver of all exposed groups after the 2 months of recovery. High levels of DNA adduct were found also in the intestines of individuals exposed to oil or heavy PAHs, but not in the PW or control groups. This suggests that the intestinal barrier is very important for detoxification of orally exposures of PAHs.

MeSH

Injections, Dna Adducts, Injections, Intraperitoneal, Recycling, Liver, Dna, Dna Damage

Cited by