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Publications

Journal Article Epublish 8 citations

PubMed: 34858689 DOI: 10.1364/BOE.439404

Label-free monitoring of 3D cortical neuronal growth using optical diffraction tomography.

Biomed Opt Express 12(11):6928-6939

Authors: Ariel J Lee, DongJo Yoon, SeungYun Han, Herve Hugonnet, WeiSun Park, Je-Kyun Park, YoonKey Nam, YongKeun Park

Affiliations

  • Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea. KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, Republic of Korea. Current Affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Contributed equally.
  • Department of Bio and Brain Engineering, KAIST, Daejeon 34141, Republic of Korea. Contributed equally.
  • Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
  • KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, Republic of Korea.
  • Current Affiliation: Department of Applied Physics, Yale University, New Haven, CT 06520, USA.
  • Contributed equally.
  • Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea. KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, Republic of Korea.
  • Department of Bio and Brain Engineering, KAIST, Daejeon 34141, Republic of Korea.
  • Department of Bio and Brain Engineering, KAIST, Daejeon 34141, Republic of Korea.
  • Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea. KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, Republic of Korea. Tomocube Inc. Daejeon, Republic of Korea.

Abstract

The highly complex central nervous systems of mammals are often studied using three-dimensional (3D) primary neuronal cultures. A coupled confocal microscopy and immunofluorescence labeling are widely utilized for visualizing the 3D structures of neurons. However, this requires fixation of the neurons and is not suitable for monitoring an identical sample at multiple time points. Thus, we propose a label-free monitoring method for 3D neuronal growth based on refractive index tomograms obtained by optical diffraction tomography. The 3D morphology of the neurons was clearly visualized, and the developmental processes of neurite outgrowth in 3D spaces were analyzed for individual neurons.

MeSH

Growth, Neurons, Tomography

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