Journal Article Epublish 3 citations
PubMed: 35008705 DOI: 10.3390/ijms23010279
Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection.
Int J Mol Sci 23(1)
Authors: Igor Buzalewicz, Iwona Hołowacz, Anna K Matczuk, Mateusz Guźniczak, Dominika Skrzela, Magdalena Karwańska, Alina Wieliczko, Katarzyna Kowal, Agnieszka Ulatowska-Jarża
Affiliations
- Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology, 27 Wybrzeże S. Wyspiańskiego St. 50-370 Wroclaw, Poland.
- Department of Pathology, Division of Microbiology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, 31 C.K. Norwida St. 51-375 Wroclaw, Poland.
- Department of Epizootiology and Veterinary Administration with Clinic of Infectious Diseases, Wrocław University of Environmental and Life Sciences, 45 Grunwaldzki Square, 50-366 Wroclaw, Poland.
- Independent Researcher, 54-104 Wroclaw, Poland.
Abstract
The worldwide increase in bacterial resistance and healthcare-associated bacterial infections pose a serious threat to human health. The antimicrobial photodynamic method reveals the opportunity for a new therapeutic approach that is based on the limited delivery of photosensitizer from the material surface. Nanoporous inorganic-organic composites were obtained by entrapment of photosensitizer Photolon in polysiloxanes that was prepared by the sol-gel method. The material was characterized by its porosity, optical properties (fluorescence and absorbance), and laser-induced antimicrobial activity against , , , and . The permanent encapsulation of Photolon in the silica coating and the antimicrobial efficiency was confirmed by confocal microscope and digital holotomography. The generation of free radicals from nanoporous surfaces was proved by scanning Kelvin probe microscopy. For the first time, it was confirmed that Kelvin probe microscopy can be a label-free, noncontact alternative to other conventional methods based on fluorescence or chemiluminescence probes, etc. It was confirmed that the proposed photoactive coating enables the antibacterial photodynamic effect based on free radicals released from the surface of the coating. The highest bactericidal efficiency of the proposed coating was 87.16%. This coating can selectively limit the multiplication of bacterial cells, while protecting the environment and reducing the risk of surface contamination.
MeSH
Microscopy, Confocal, Photosensitizing Agents, Microscopy, Free Radicals