Journal Article Research Support, Non-U.S. Gov't Epublish 12 citations
PubMed: 35205342 DOI: 10.3390/genes13020298
Kisspeptin-10 Promotes Progesterone Synthesis in Bovine Ovarian Granulosa Cells via Downregulation of microRNA-1246.
Genes (Basel) 13(2)
Authors: Lewei Guo, Haoran Xu, Yajun Li, Hongyu Liu, Jing Zhao, Wenfa Lu, Jun Wang
Affiliations
- Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
- College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Abstract
The objective of this study was to clarify the effect of kisspeptin-10 (kp-10) on the synthesis of progesterone (P4) in bovine granulosa cells (BGCs) and its mechanisms via microRNA 1246 (miR-1246). According to the results, we found that treating with kp-10 for 24 h could increase P4 level, the mRNA expression of the steroidogenesis-related gene (StAR), free cholesterol content, and decrease miR-1246 expression in BGCs. Overexpression of miR-1246 significantly inhibited P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs, whereas underexpression of miR-1246 significantly reversed this effect in BGCs. Additionally, overexpression of miR-1246 counteracted the accelerative effect of kp-10 on P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs. Conversely, underexpression of miR-1246 enhanced the accelerative effect of kp-10 on P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs. Meanwhile, results of dual-luciferase reporter assays indicated that miR-1246 targeted the 3'UTR of StAR in BGCs. These results demonstrated that kp-10 induced P4 synthesis in BGCs by promoting free cholesterol transport via regulating expression of miR-1246/StAR.
MeSH
Progesterone, Cholesterol, Granulosa Cells, Genes, Genomics, 3' Untranslated Regions, Micrornas, Down-Regulation, Kisspeptins
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- miR-202-5p Inhibits Lipid Metabolism and Steroidogenesis of Goose Hierarchical Granulosa Cells by Targeting ACSL3. Animals (Basel), 2023
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