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Publications

Journal Article Ppublish 75 citations

PubMed: 35540496 DOI: 10.1038/s43586-020-00006-x

Recombineering and MAGE.

Nat Rev Methods Primers 1

Authors: Timothy M Wannier, Peter N Ciaccia, Andrew D Ellington, Gabriel T Filsinger, Farren J Isaacs, Kamyab Javanmardi, Michaela A Jones, Aditya M Kunjapur, Akos Nyerges, Csaba Pal, Max G Schubert, George M Church

Affiliations

  • Department of Genetics, Harvard Medical School, Boston, MA, USA.
  • Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
  • Department of Molecular, Cellular & Developmental Biology, Yale University, New Haven, CT, USA. Systems Biology Institute, Yale University, West Haven, CT, USA.
  • Department of Molecular Biosciences, College of Natural Sciences, University of Texas at Austin, Austin, TX, USA.
  • Department of Systems Biology, Harvard University, Cambridge, MA, USA.
  • Department of Molecular, Cellular & Developmental Biology, Yale University, New Haven, CT, USA.
  • Systems Biology Institute, Yale University, West Haven, CT, USA.
  • Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
  • Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
  • Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.

Abstract

Recombination-mediated genetic engineering, also known as recombineering, is the genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial genomes. Recombineering and its derivative methods have radically improved genome engineering capabilities, perhaps none more so than multiplex automated genome engineering (MAGE). MAGE is representative of a set of highly multiplexed single-stranded DNA-mediated technologies. First described in , both MAGE and recombineering are being rapidly translated into diverse prokaryotes and even into eukaryotic cells. Together, this modern set of tools offers the promise of radically improving the scope and throughput of experimental biology by providing powerful new methods to ease the genetic manipulation of model and non-model organisms. In this Primer, we describe recombineering and MAGE, their optimal use, their diverse applications and methods for pairing them with other genetic editing tools. We then look forward to the future of genetic engineering.

MeSH

Genetic Engineering, Dna, Single-Stranded, Genome

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