Journal Article Epublish 8 citations
PubMed: 35562904 DOI: 10.3390/ijms23094512
Morphometric and Nanomechanical Features of Erythrocytes Characteristic of Early Pregnancy Loss.
Int J Mol Sci 23(9)
Authors: Ariana Langari, Velichka Strijkova, Regina Komsa-Penkova, Avgustina Danailova, Sashka Krumova, Stefka G Taneva, Ina Giosheva, Emil Gartchev, Kamelia Kercheva, Alexey Savov, Svetla Todinova
Affiliations
- Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, "Acad. G. Bontchev" Str. 21, 1113 Sofia, Bulgaria.
- Institute of Optical Materials and Technologies "Acad. Yordan Malinovski", Bulgarian Academy of Sciences, "Acad. G. Bontchev" Str. 109, 1113 Sofia, Bulgaria.
- Department of Biochemistry, Medical University-Pleven, Sv. Kliment Ohridski Str. 1, 5800 Pleven, Bulgaria.
- University Hospital of Obstetrics and Gynecology "Maichin Dom", Medical University Sofia, Zdrave Str. 2, 1431 Sofia, Bulgaria.
Abstract
Early pregnancy loss (EPL) is estimated to be between 15 and 20% of all adverse pregnancies. Approximately, half of EPL cases have no identifiable cause. Herein, we apply atomic force microscopy to evaluate the alteration of morphology and nanomechanics of erythrocytes from women with EPL with unknown etiology, as compared to healthy pregnant (PC) and nonpregnant women (NPC). Freshly isolated erythrocytes from women with EPL differ in both the roughness value (4.6 ± 0.3 nm, p < 0.05), and Young’s modulus (2.54 ± 0.6 MPa, p < 0.01) compared to the values for NPC (3.8 ± 0.4 nm and 0.94 ± 0.2 MPa, respectively) and PC (3.3 ± 0.2 nm and 1.12 ± 0.3 MPa, respectively). Moreover, we find a time-dependent trend for the reduction of the cells’ morphometric parameters (cells size and surface roughness) and the membrane elasticity—much faster for EPL than for the two control groups. The accelerated aging of EPL erythrocytes is expressed in faster morphological shape transformation and earlier occurrence of spiculated and spherical-shaped cells, reduced membrane roughness and elasticity with aging evolution. Oxidative stress in vitro contributed to the morphological cells’ changes observed for EPL senescent erythrocytes. The ultrastructural characteristics of cells derived from women with miscarriages show potential as a supplementary mark for a pathological state.
MeSH
Cells, Pregnancy, Microscopy, Atomic Force, Erythrocytes, Aging, Abortion, Spontaneous, Oxidative Stress, Membranes