Journal Article Research Support, Non-U.S. Gov't Epublish 11 citations
PubMed: 35563714 DOI: 10.3390/cells11091408
Human Wharton's Jelly Mesenchymal Stem Cells Secretome Inhibits Human SARS-CoV-2 and Avian Infectious Bronchitis Coronaviruses.
Cells 11(9)
Authors: Mohamed A A Hussein, Hosni A M Hussein, Ali A Thabet, Karim M Selim, Mervat A Dawood, Ahmed M El-Adly, Ahmed A Wardany, Ali Sobhy, Sameh Magdeldin, Aya Osama, Ali M Anwar, Mohammed Abdel-Wahab, Hussam Askar, Elsayed K Bakhiet, Serageldeen Sultan, Amgad A Ezzat, Usama Abdel Raouf, Magdy M Afifi
Affiliations
- Department of Microbiology, Faculty of Science, Al-Azhar University, Assiut 71524, Egypt.
- Department of Zoology, Faculty of Science, Al-Azhar University, Assiut 71524, Egypt.
- Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center, Dokki, Giza 12618, Egypt.
- Clinical Pathology, Mansoura Research Center for Cord Stem Cells (MARC-CSC), Faculty of Medicine, Mansoura University, El Mansoura 35516, Egypt.
- Department of Clinical Pathology, Faculty of Medicine, Al-Azhar University, Assiut 71524, Egypt.
- Proteomics and Metabolomics Research Program, Basic Research Department, Children's Cancer Hospital, (CCHE-57357), Cairo 57357, Egypt.
- Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
- Department of Microbiology, Virology Division, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
- Department of Medical Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Assiut 71524, Egypt.
- Department of Botany and Microbiology, Faculty of Science, Aswan University, Aswan 81528, Egypt.
Abstract
Human SARS-CoV-2 and avian infectious bronchitis virus (IBV) are highly contagious and deadly coronaviruses, causing devastating respiratory diseases in humans and chickens. The lack of effective therapeutics exacerbates the impact of outbreaks associated with SARS-CoV-2 and IBV infections. Thus, novel drugs or therapeutic agents are highly in demand for controlling viral transmission and disease progression. Mesenchymal stem cells (MSC) secreted factors (secretome) are safe and efficient alternatives to stem cells in MSC-based therapies. This study aimed to investigate the antiviral potentials of human Wharton’s jelly MSC secretome (hWJ-MSC-S) against SARS-CoV-2 and IBV infections in vitro and in ovo. The half-maximal inhibitory concentrations (IC50), cytotoxic concentration (CC50), and selective index (SI) values of hWJ-MSC-S were determined using Vero-E6 cells. The virucidal, anti-adsorption, and anti-replication antiviral mechanisms of hWJ-MSC-S were evaluated. The hWJ-MSC-S significantly inhibited infection of SARS-CoV-2 and IBV, without affecting the viability of cells and embryos. Interestingly, hWJ-MSC-S reduced viral infection by >90%, in vitro. The IC50 and SI of hWJ-MSC secretome against SARS-CoV-2 were 166.6 and 235.29 µg/mL, respectively, while for IBV, IC50 and SI were 439.9 and 89.11 µg/mL, respectively. The virucidal and anti-replication antiviral effects of hWJ-MSC-S were very prominent compared to the anti-adsorption effect. In the in ovo model, hWJ-MSC-S reduced IBV titer by >99%. Liquid chromatography-tandem mass spectrometry (LC/MS-MS) analysis of hWJ-MSC-S revealed a significant enrichment of immunomodulatory and antiviral proteins. Collectively, our results not only uncovered the antiviral potency of hWJ-MSC-S against SARS-CoV-2 and IBV, but also described the mechanism by which hWJ-MSC-S inhibits viral infection. These findings indicate that hWJ-MSC-S could be utilized in future pre-clinical and clinical studies to develop effective therapeutic approaches against human COVID-19 and avian IB respiratory diseases.
MeSH
Sars-Cov-2, Infectious Bronchitis Virus, Microscopy, Electron, Scanning Transmission, Mesenchymal Stem Cells, Covid-19, Wharton Jelly, Viruses, Therapeutics, Severe Acute Respiratory Syndrome-Related Coronavirus, Antiviral Agents, Infections, Virus Diseases
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