Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural Epublish 6 citations
PubMed: 35713400 DOI: 10.7554/eLife.76497
Functional requirements for a Samd14-capping protein complex in stress erythropoiesis.
Elife 11
Authors: Suhita Ray, Linda Chee, Yichao Zhou, Meg A Schaefer, Michael J Naldrett, Sophie Alvarez, Nicholas T Woods, Kyle J Hewitt
Affiliations
- Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, United States.
- Proteomics and Metabolomics Facility, University of Nebraska-Lincoln, Lincoln, United States.
- Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, United States.
Abstract
Acute anemia induces rapid expansion of erythroid precursors and accelerated differentiation to replenish erythrocytes. Paracrine signals-involving cooperation between stem cell factor (SCF)/Kit signaling and other signaling inputs-are required for the increased erythroid precursor activity in anemia. Our prior work revealed that the sterile alpha motif (SAM) domain 14 () gene increases the regenerative capacity of the erythroid system in a mouse genetic model and promotes stress-dependent Kit signaling. However, the mechanism underlying Samd14's role in stress erythropoiesis is unknown. We identified a protein-protein interaction between Samd14 and the α- and β-heterodimers of the F-actin capping protein (CP) complex. Knockdown of the CP β subunit increased erythroid maturation in murine ex vivo cultures and decreased colony forming potential of stress erythroid precursors. In a genetic complementation assay for Samd14 activity, our results revealed that the Samd14-CP interaction is a determinant of erythroid precursor cell levels and function. Samd14-CP promotes SCF/Kit signaling in CD71 spleen erythroid precursors. Given the roles of Kit signaling in hematopoiesis and Samd14 in Kit pathway activation, this mechanism may have pathological implications in acute/chronic anemia.
MeSH
Biological Science Disciplines, Erythropoietin, Stress, Physiological, Erythropoiesis, Anemia, Stem Cells, Microscopy, Electron, Scanning Transmission, Genes, Regenerative Medicine, Cell Biology
Cited by
- The SAMD1 transcription factor coordinates hematopoietic lineage differentiation and H3K4 methylation status. Blood Adv, 2025
- Physiological and regenerative functions of sterile-α motif protein-14 in hematopoiesis. Exp Hematol, 2023
- Genome wide association study reveals novel associations with face morphology. PLoS One, 2025
- Signaling mechanisms and cis -regulatory control of Samd14 in erythroid regeneration. Curr Opin Hematol, 2025