Journal Article Research Support, Non-U.S. Gov't Epublish 28 citations
PubMed: 35752733 DOI: 10.1038/s41467-022-31438-6
FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis.
Nat Commun 13(1):3644
Authors: Stuart D Desjardins, James Simmonds, Inna Guterman, Kostya Kanyuka, Amanda J Burridge, Andrew J Tock, Eugenio Sanchez-Moran, F Chris H Franklin, Ian R Henderson, Keith J Edwards, Cristobal Uauy, James D Higgins
Affiliations
- Department of Genetics and Genome Biology, Adrian Building, University of Leicester, University Road, Leicester, LE1 7RH, UK.
- Department of Crop Genetics, John Innes Centre, Norwich, NR4 7UH, UK.
- NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK.
- Biointeractions and Crop Protection, Rothamsted Research, Harpenden, AL5 2JQ, UK.
- Life Sciences Building, University of Bristol, 24 Tyndall Avenue, Bristol, BS8 1TQ, UK.
- Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, CB2 3EA, UK.
- School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK.
- Department of Genetics and Genome Biology, Adrian Building, University of Leicester, University Road, Leicester, LE1 7RH, UK.
Abstract
FANCM suppresses crossovers in plants by unwinding recombination intermediates. In wheat, crossovers are skewed toward the chromosome ends, thus limiting generation of novel allelic combinations. Here, we observe that FANCM maintains the obligate crossover in tetraploid and hexaploid wheat, thus ensuring that every chromosome pair exhibits at least one crossover, by localizing class I crossover protein HEI10 at pachytene. FANCM also suppresses class II crossovers that increased 2.6-fold in fancm msh5 quadruple mutants. These data are consistent with a role for FANCM in second-end capture of class I designated crossover sites, whilst FANCM is also required to promote formation of non-crossovers. In hexaploid wheat, genetic mapping reveals that crossovers increase by 31% in fancm compared to wild type, indicating that fancm could be an effective tool to accelerate breeding. Crossover rate differences in fancm correlate with wild type crossover distributions, suggesting that chromatin may influence the recombination landscape in similar ways in both wild type and fancm.
MeSH
Recombination, Genetic, Chromosomes, Meiosis, Exhibitions As Topic
Cited by
- The DNA helicase FANCJ (BRIP1) functions in double strand break repair processing, but not crossover formation during prophase I of meiosis in male mice. PLoS Genet, 2024
- Genetic dissection of MutL complexes in Arabidopsis meiosis. Nucleic Acids Res, 2025
- Unravelling mechanisms that govern meiotic crossover formation in wheat. Biochem Soc Trans, 2022
- Gross Chromosomal Rearrangement at Centromeres. Biomolecules, 2024
- Wheat improvement through advances in single nucleotide polymorphism (SNP) detection and genotyping with a special emphasis on rust resistance. Theor Appl Genet, 2024
- Characterization and acceleration of genome shuffling and ploidy reduction in synthetic allopolyploids by genome sequencing and editing. Nucleic Acids Res, 2023
- The DNA helicase FANCJ (BRIP1) functions in Double Strand Break repair processing, but not crossover formation during Prophase I of meiosis in male mice. bioRxiv, 2023
- contributes to maintain both homologous and homoeologous recombination during wheat meiosis. Front Plant Sci, 2024