Journal Article Epublish 99 citations
PubMed: 35790748 DOI: 10.1038/s41377-022-00898-2
Quantitative phase imaging through an ultra-thin lensless fiber endoscope.
Light Sci Appl 11(1):204
Authors: Jiawei Sun, Jiachen Wu, Song Wu, Ruchi Goswami, Salvatore Girardo, Liangcai Cao, Jochen Guck, Nektarios Koukourakis, Juergen W Czarske
Affiliations
- Laboratory of Measurement and Sensor System Technique (MST), TU Dresden, Helmholtzstrasse 18, 01069, Dresden, Germany. Competence Center for Biomedical Computational Laser Systems (BIOLAS), TU Dresden, Dresden, Germany.
- Laboratory of Measurement and Sensor System Technique (MST), TU Dresden, Helmholtzstrasse 18, 01069, Dresden, Germany. State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, 100084, Beijing, China.
- Institute for Integrative Nanosciences, IFW Dresden, Helmholtzstraße 20, 01069, Dresden, Germany.
- Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058, Erlangen, Germany.
- State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, 100084, Beijing, China.
- Cluster of Excellence Physics of Life, TU Dresden, Dresden, Germany.
- Laboratory of Measurement and Sensor System Technique (MST), TU Dresden, Helmholtzstrasse 18, 01069, Dresden, Germany.
- Competence Center for Biomedical Computational Laser Systems (BIOLAS), TU Dresden, Dresden, Germany.
- Laboratory of Measurement and Sensor System Technique (MST), TU Dresden, Helmholtzstrasse 18, 01069, Dresden, Germany.
- Competence Center for Biomedical Computational Laser Systems (BIOLAS), TU Dresden, Dresden, Germany.
- Cluster of Excellence Physics of Life, TU Dresden, Dresden, Germany.
- Institute of Applied Physics, TU Dresden, Dresden, Germany.
Abstract
Quantitative phase imaging (QPI) is a label-free technique providing both morphology and quantitative biophysical information in biomedicine. However, applying such a powerful technique to in vivo pathological diagnosis remains challenging. Multi-core fiber bundles (MCFs) enable ultra-thin probes for in vivo imaging, but current MCF imaging techniques are limited to amplitude imaging modalities. We demonstrate a computational lensless microendoscope that uses an ultra-thin bare MCF to perform quantitative phase imaging with microscale lateral resolution and nanoscale axial sensitivity of the optical path length. The incident complex light field at the measurement side is precisely reconstructed from the far-field speckle pattern at the detection side, enabling digital refocusing in a multi-layer sample without any mechanical movement. The accuracy of the quantitative phase reconstruction is validated by imaging the phase target and hydrogel beads through the MCF. With the proposed imaging modality, three-dimensional imaging of human cancer cells is achieved through the ultra-thin fiber endoscope, promising widespread clinical applications.
MeSH
Endoscopes, Quantitative Phase Imaging
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