Journal Article Research Support, Non-U.S. Gov't Epublish 2 citations
PubMed: 35982897 DOI: 10.7150/ijbs.75084
The Novel Role of hnRNP UL1 in Human Cell Nucleoli.
Int J Biol Sci 18(13):4809-4823
Authors: Marlena Cichocka, Anna Karlik, Patrycja Plewka, Kishor Gawade, Agata Stępień, Patrycja Świergiel, Ankur Gadgil, Katarzyna Dorota Raczyńska
Affiliations
- Laboratory of RNA Processing, Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Uniwersytetu Poznańskiego 6, 61-614 Poznan, Poland. Center for Advanced Technology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 10, 61-614 Poznan, Poland.
- Laboratory of RNA Processing, Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Uniwersytetu Poznańskiego 6, 61-614 Poznan, Poland.
- Center for Advanced Technology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 10, 61-614 Poznan, Poland.
Abstract
hnRNP UL1 plays an important role in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double-strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is also localized in the nucleoli of human cells. Upon investigating its function, we found that hnRNP UL1 stimulates ribosomal DNA (rDNA) gene transcription. Moreover, we observed that cells with hnRNP UL1 silencing exhibited increased sensitivity to DNA damage. We also showed that hnRNP UL1 interacts with γH2A.X, RPA32, XRCC1, and Chk1 in cell nucleoli, suggesting its involvement in the repair of rDNA damage.
MeSH
Biological Science Disciplines, Cells, Cell Nucleolus, Genes, Heterogeneous-Nuclear Ribonucleoproteins, Dna, Ribosomal, Rna Polymerase Ii