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Journal Article Review Research Support, Non-U.S. Gov't Epublish 63 citations

PubMed: 36153611 DOI: 10.1186/s13148-022-01337-0

Liquid biopsies based on DNA methylation as biomarkers for the detection and prognosis of lung cancer.

Clin Epigenetics 14(1):118

Authors: Peilong Li, Shibiao Liu, Lutao Du, Ghazal Mohseni, Yi Zhang, Chuanxin Wang

Affiliations

  • Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250033, Shandong, China. Shandong Engineering and Technology Research Center for Tumor Marker Detection, Jinan, Shandong, China.
  • Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250033, Shandong, China.
  • Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250033, Shandong, China. Shandong Engineering and Technology Research Center for Tumor Marker Detection, Jinan, Shandong, China. Shandong Provincial Clinical Medicine Research Center for Clinical Laboratory, Jinan, Shandong, China.
  • Department of Respiratory and Critical Care Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, China.
  • Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250033, Shandong, China. Shandong Engineering and Technology Research Center for Tumor Marker Detection, Jinan, Shandong, China. Shandong Provincial Clinical Medicine Research Center for Clinical Laboratory, Jinan, Shandong, China.

Abstract

Lung cancer (LC) is the main cause of cancer-related mortality. Most LC patients are diagnosed in an advanced stage when the symptoms are obvious, and the prognosis is quite poor. Although low-dose computed tomography (LDCT) is a routine clinical examination for early detection of LC, the false-positive rate is over 90%. As one of the intensely studied epigenetic modifications, DNA methylation plays a key role in various diseases, including cancer and other diseases. Hypermethylation in tumor suppressor genes or hypomethylation in oncogenes is an important event in tumorigenesis. Remarkably, DNA methylation usually occurs in the very early stage of malignant tumors. Thus, DNA methylation analysis may provide some useful information about the early detection of LC. In recent years, liquid biopsy has developed rapidly. Liquid biopsy can detect and monitor both primary and metastatic malignant tumors and can reflect tumor heterogeneity. Moreover, it is a minimally invasive procedure, and it causes less pain for patients. This review summarized various liquid biopsies based on DNA methylation for LC. At first, we briefly discussed some emerging technologies for DNA methylation analysis. Subsequently, we outlined cell-free DNA (cfDNA), sputum, bronchoalveolar lavage fluid, bronchial aspirates, and bronchial washings DNA methylation-based liquid biopsy for the early detection of LC. Finally, the prognostic value of DNA methylation in cfDNA and sputum and the diagnostic value of other DNA methylation-based liquid biopsies for LC were also analyzed.

MeSH

Sputum, Prognosis, Biomarkers, Lung Neoplasms, Dna Methylation, Epigenesis, Genetic, Genes, Liquid Biopsy

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