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Publications

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Ppublish 11 citations

PubMed: 36239844 DOI: 10.1007/s11248-022-00328-4

An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation.

Transgenic Res 31(6):661-676

Authors: Mónica Prías-Blanco, Timothy M Chappell, Emily F Freed, Eudald Illa-Berenguer, Carrie A Eckert, Wayne A Parrott

Affiliations

  • Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, USA.
  • Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, Boulder, CO, USA.
  • Center for Applied Genetic Technologies, University of Georgia, Athens, GA, USA.
  • Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
  • Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, USA.
  • Center for Applied Genetic Technologies, University of Georgia, Athens, GA, USA.
  • Department of Crop and Soil Sciences, University of Georgia, Athens, GA, USA.

Abstract

Auxotrophic strains of Agrobacterium tumefaciens can contribute to the development of more efficient transformation systems, especially for crops historically considered recalcitrant. Homologous recombination was used to derive methionine auxotrophs of two common A. tumefaciens strains, LBA4404 and EHA105. The EHA105 strains were more efficient for switchgrass transformation, while both the EHA105 and LBA4404 strains worked equally well for the rice control. Event quality, as measured by transgene copy number, was not affected by auxotrophy, but was higher for the LBA4404 strains than the EHA105 strains. Ultimately, the use of auxotrophs reduced bacterial overgrowth during co-cultivation and decreased the need for antibiotics.

MeSH

Methionine, Plants, Genetically Modified, Agrobacterium

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