Journal Article Epublish 1 citation
PubMed: 36432849 DOI: 10.3390/plants11223119
Response of Makino Plants to Heat Stress and Selection of Superior Clones Using Morphological and Molecular Analysis.
Plants (Basel) 11(22)
Authors: Hyung-Eun Kim, Jong-Eun Han, Hosakatte Niranjana Murthy, Hyuk-Joon Kwon, Gun-Myung Lee, So-Young Park
Affiliations
- Department of Horticultural Science, Chungbuk National University, Cheongju 28644, Republic of Korea. Korea Disease Control and Prevention Agency, Osong-eup, Cheongju 28159, Republic of Korea.
- Department of Horticultural Science, Chungbuk National University, Cheongju 28644, Republic of Korea.
- Department of Horticultural Science, Chungbuk National University, Cheongju 28644, Republic of Korea. Department of Botany, Karnatak University, Dharwad 580003, India.
- Food Science R&D Center, Kolmar BNH Co. Seocho-gu, Seoul 30003, Republic of Korea.
Abstract
is a medicinal plant cultivated for its rhizomes, which are used in Chinese, Japanese, and Korean traditional medicine. This medicinal crop is highly susceptible to heat stress and cannot be cultivated in regions of higher temperatures. In the present study, ten clones from Korea (clones 1, 2, 5, 6, 8, 11, 14, 15, 22, and 26) were evaluated for their heat tolerance in vitro at 25, 30, 32.5, and 35 °C, and growth characteristics including plant height, the number of leaves and roots were evaluated. The initial experiment was conducted to find the threshold level for significant damage to the plant, while the second experiment was to screen the germplasm to select heat-tolerant clones. Most of the clones were sensitive to heat stress (clones 1, 2, 8, 11, 14, 15, 22, and 26), and few clones (clones 5 and 6) could perform well at an elevated temperature of 32.5 °C. Molecular analysis of the expression of heat-responsive genes, including heat shock protein (), catalase (), and cystine protease (), was performed by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) carried out with heat-tolerant and heat-sensitive clones. Two of the heat-tolerant clones (clones 5 and 6) showed significant expression of and genes at elevated temperature treatment. These clones can be used for further evaluation and cultivation.
MeSH
Heat Stress Disorders, Stress, Physiological, Clone Cells, Catalase, Temperature, Genes, Heat-Shock Proteins, Polymerase Chain Reaction