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Publications

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Epublish 51 citations

PubMed: 36463216 DOI: 10.1038/s41467-022-35034-6

Membrane curvature governs the distribution of Piezo1 in live cells.

Nat Commun 13(1):7467

Authors: Shilong Yang, Xinwen Miao, Steven Arnold, Boxuan Li, Alan T Ly, Huan Wang, Matthew Wang, Xiangfu Guo, Medha M Pathak, Wenting Zhao, Charles D Cox, Zheng Shi

Affiliations

  • Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ, 08854, USA.
  • School of Chemistry, Chemical Engineering, and Biotechnology, Nanyang Technological University, 637457, Singapore, Singapore.
  • Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, 08854, USA.
  • Department of Physiology & Biophysics, UC Irvine, Irvine, CA, 92697, USA.
  • Sue and Bill Gross Stem Cell Research Center, UC Irvine, Irvine, CA, 92697, USA.
  • Department of Physics, Rutgers University, Piscataway, NJ, 08854, USA.
  • Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Sydney, NSW, Australia.
  • Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ, 08854, USA. Cancer Pharmacology Research Program, Cancer Institute of New Jersey, Rutgers University, New Brunswick, NJ, 08901, USA.

Abstract

Piezo1 is a bona fide mechanosensitive ion channel ubiquitously expressed in mammalian cells. The distribution of Piezo1 within a cell is essential for various biological processes including cytokinesis, cell migration, and wound healing. However, the underlying principles that guide the subcellular distribution of Piezo1 remain largely unexplored. Here, we demonstrate that membrane curvature serves as a key regulator of the spatial distribution of Piezo1 in the plasma membrane of living cells. Piezo1 depletes from highly curved membrane protrusions such as filopodia and enriches to nanoscale membrane invaginations. Quantification of the curvature-dependent sorting of Piezo1 directly reveals the in situ nano-geometry of the Piezo1-membrane complex. Piezo1 density on filopodia increases upon activation, independent of calcium, suggesting flattening of the channel upon opening. Consequently, the expression of Piezo1 inhibits filopodia formation, an effect that diminishes with channel activation.

MeSH

Biological Science Disciplines, Membranes, Cells

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