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Journal Article Research Support, Non-U.S. Gov't Ppublish 14 citations

PubMed: 36511706 DOI: 10.1128/msphere.00581-22

Gut-Spleen Axis: Microbiota via Vascular and Immune Pathways Improve Busulfan-Induced Spleen Disruption.

mSphere 8(1):e0058122

Authors: Hanhan Fang, Xiaohui Feng, Tao Xu, Ruqing Zhong, Dongxin Lu, Hongfu Zhang, Wei Shen, Yong Zhao, Liang Chen, Junjie Wang

Affiliations

  • College of Life Sciences, Qingdao Agricultural University, Qingdao, People's Republic of China. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
  • College of Life Sciences, Qingdao Agricultural University, Qingdao, People's Republic of China.
  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China. College of Science, Health, Engineering and Education, Murdoch University, Perth, Australia.
  • College of Science, Health, Engineering and Education, Murdoch University, Perth, Australia.

Abstract

Fecal microbiota transplantation (FMT) is an effective means of modulating gut microbiota for the treatment of many diseases, including Clostridioides difficile infections. The gut-spleen axis has been established, and this is involved in the development and function of the spleen. However, it is not understood whether gut microbiota can be used to improve spleen function, especially in spleens disrupted by a disease or an anti-cancer treatment. In the current investigation, we established that alginate oligosaccharide (AOS)-improved gut microbiota (A10-FMT) can rescue anticancer drug busulfan-disrupted spleen vasculature and spleen function. A10-FMT improved the gene and/or protein expression of genes involved in vasculature development, increased the cell proliferation rate, enhanced the endothelial progenitor cell capability, and elevated the expression of the cell junction molecules to increase the vascularization of the spleen. This investigation found for the first time that the reestablishment of spleen vascularization restored spleen function by improving spleen immune cells and iron metabolism. These findings may be used as a strategy to minimize the side effects of anti-cancer drugs or to improve spleen vasculature-related diseases. Alginate oligosaccharide (AOS)-improved gut microbiota (A10-FMT) can rescue busulfan disrupted spleen vasculature. A10-FMT improved the cell proliferation rate, endothelial progenitor cell capability, and cell junction molecules to increase vasculature formation in the spleen. This reestablishment restored spleen function by improving spleen immune cells and iron metabolism. These findings are useful for the treatment of spleen vasculature-related diseases.

MeSH

Microbiological Phenomena, Microbiota, Pharmaceutical Preparations, Busulfan, Stem Cells, Neoplasms, Intercellular Junctions, Cell Proliferation, Spleen, Genes

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