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Publications

Journal Article Research Support, Non-U.S. Gov't Epublish 27 citations

PubMed: 36537079 DOI: 10.7554/eLife.81316

Cross-talk between red blood cells and plasma influences blood flow and omics phenotypes in severe COVID-19.

Elife 11

Authors: Steffen M Recktenwald, Greta Simionato, Marcelle G M Lopes, Fabia Gamboni, Monika Dzieciatkowska, Patrick Meybohm, Kai Zacharowski, Andreas von Knethen, Christian Wagner, Lars Kaestner, Angelo D'Alessandro, Stephan Quint

Affiliations

  • Dynamics of Fluids, Department of Experimental Physics, Saarland University, Saarbrücken, Germany.
  • Institute for Clinical and Experimental Surgery, Campus University Hospital, Saarland University, Homburg, Germany.
  • Cysmic GmbH, Saarbrücken, Germany.
  • Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, United States.
  • Department of Anesthesiology, Intensive Care, Emergency and Pain Medicine, University Hospital Wuerzburg, Wuerzburg, Germany.
  • Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, University Hospital Frankfurt, Frankfurt, Germany.
  • Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Frankfurt, Germany.
  • Department of Physics and Materials Science, University of Luxembourg, Luxembourg City, Luxembourg.
  • Theoretical Medicine and Biosciences, Campus University Hospital, Saarland University, Homburg, Germany.

Abstract

Coronavirus disease 2019 (COVID-19) is caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and can affect multiple organs, among which is the circulatory system. Inflammation and mortality risk markers were previously detected in COVID-19 plasma and red blood cells (RBCs) metabolic and proteomic profiles. Additionally, biophysical properties, such as deformability, were found to be changed during the infection. Based on such data, we aim to better characterize RBC functions in COVID-19. We evaluate the flow properties of RBCs in severe COVID-19 patients admitted to the intensive care unit by using microfluidic techniques and automated methods, including artificial neural networks, for an unbiased RBC analysis. We find strong flow and RBC shape impairment in COVID-19 samples and demonstrate that such changes are reversible upon suspension of COVID-19 RBCs in healthy plasma. Vice versa, healthy RBCs resemble COVID-19 RBCs when suspended in COVID-19 plasma. Proteomics and metabolomics analyses allow us to detect the effect of plasma exchanges on both plasma and RBCs and demonstrate a new role of RBCs in maintaining plasma equilibria at the expense of their flow properties. Our findings provide a framework for further investigations of clinical relevance for therapies against COVID-19 and possibly other infectious diseases.

MeSH

Cells, Sars-Cov-2, Erythrocytes, Blood, Biological Science Disciplines, Phenotype, Microfluidics, Cell Biology, Covid-19, Blood Cells, Blood Circulation, Plasma

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