Journal Article Epublish 8 citations
PubMed: 36556312 DOI: 10.3390/life12121947
Wound Healing and Anti-Inflammatory Effects of a Newly Developed Ointment Containing Jujube Leaves Extract.
Life (Basel) 12(12)
Authors: Marilena-Viorica Hovaneț, Emma Adriana Ozon, Elena Moroșan, Oana Cristina Șeremet, Eliza Oprea, Elisabeta-Irina Geană, Adriana Iuliana Anghel, Carmellina Bădiceanu, Ligia Elena Duțu, Cristina Silvia Stoicescu, Eugenia Nagoda, Robert Ancuceanu
Affiliations
- Department of Pharmaceutical Botany and Cell Biology, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
- Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
- Department of Clinical Laboratory and Food Safety, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
- Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
- Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 060101 Bucharest, Romania.
- National R&D Institute for Cryogenics and Isotopic Technologies-ICIT, 4th Uzinei Street, 240050 Râmnicu Vâlcea, Romania.
- Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
- Department of Pharmacognosy, Phytochemistry and Phytotherapy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
- Ilie Murgulescu Institute of Physical Chemistry, Spl. Independentei 202, P.O. Box 12-194, 060021 Bucharest, Romania.
- Botanical Garden "D. Brandza", University of Bucharest, Şos. Cotroceni 32, 060114 Bucharest, Romania.
Abstract
Mill. (jujube) is a well-known medicinal plant with pronounced wound healing properties. The present study aimed to establish the chemical composition of the lyophilized ethanolic extract from Romanian leaves and to evaluate the healing and anti-inflammatory properties of a newly developed lipophilic ointment containing 10% dried jujube leaves extract. The ultra-High-Performance Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry method was used, and 47 compounds were detected, among them the novel epicatechin and caffeic acid. The extract contains significant amounts of rutin (29.836 mg/g), quercetin (15.180 mg/g) and chlorogenic acid (350.96 µg/g). The lipophilic ointment has a slightly tolerable pH, between 5.41-5.42, and proved to be non-toxic in acute dermal irritation tests on New Zealand albino rabbits and after repeated administration on Wistar rats. The ointment also has a healing activity comparable to Cicatrizin (a pharmaceutical marketed product) on Wistar rats and a moderate anti-inflammatory action compared to the control group, but statistically insignificant compared to indomethacin in the rat-induced inflammation test by intraplantar administration of kaolin. The healing and anti-inflammatory properties of the tested ointment are due to phenolic acids and flavonoids content, less because of minor components as apocynin, scopoletin, and isofraxidin.
MeSH
Ointments, Quercetin, Chlorogenic Acid, Rutin