Journal Article Epublish 11 citations
PubMed: 36589574 DOI: 10.1364/BOE.465066
High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching.
Biomed Opt Express 13(12):6404-6415
Authors: Dohyeon Lee, Moosung Lee, Haechan Kwak, Young Seo Kim, Jaehyu Shim, Jik Han Jung, Wei-Sun Park, Ji-Ho Park, Sumin Lee, YongKeun Park
Affiliations
- Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea. KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, Republic of Korea.
- KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, Republic of Korea. Department of Bio and Brain Engineering, KAIST, Daejeon 34141, Republic of Korea.
- KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, Republic of Korea. Department of Bio and Brain Engineering, KAIST, Daejeon 34141, Republic of Korea.
- Tomocube Inc. Daejeon 34109, Republic of Korea.
- Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea. KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, Republic of Korea. Tomocube Inc. Daejeon 34109, Republic of Korea.
Abstract
Optical diffraction tomography (ODT) enables the three-dimensional (3D) refractive index (RI) reconstruction. However, when the RI difference between a sample and a medium increases, the effects of light scattering become significant, preventing the acquisition of high-quality and accurate RI reconstructions. Herein, we present a method for high-fidelity ODT by introducing non-toxic RI matching media. Optimally reducing the RI contrast enhances the fidelity and accuracy of 3D RI reconstruction, enabling visualization of the morphology and intra-organization of live biological samples without producing toxic effects. We validate our method using various biological organisms, including C. albicans and C. elegans.
MeSH
Tomography
Cited by
- Optical diffraction tomography for assessing single cell models in angular light scattering. Biomed Opt Express, 2024
- Holotomography and atomic force microscopy: a powerful combination to enhance cancer, microbiology and nanotoxicology research. Discov Nano, 2024
- A Non-Invasive, Label-Free Method for Examining Tardigrade Anatomy Using Holotomography. Tomography, 2025
- Multiplexed Chromatin Analysis Using Optical Spectroscopic Statistical Nanosensing. ACS Photonics, 2025
- Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy. Sensors (Basel), 2024