Journal Article Epublish 16 citations
PubMed: 36614250 DOI: 10.3390/ijms24010807
Transcriptome Analysis of the Effects of Grafting Interstocks on Apple Rootstocks and Scions.
Int J Mol Sci 24(1)
Authors: Qingshan Li, Yuan Gao, Kun Wang, Jianrong Feng, Simiao Sun, Xiang Lu, Zhao Liu, Deying Zhao, Lianwen Li, Dajiang Wang
Affiliations
- Key Laboratory of Horticulture Crops Germplasm Resources Utilization, Research Institute of Pomology, Chinese Academy of Agricultural Sciences (CAAS), Ministry of Agriculture and Rural Affairs of the People's Republic of China, No. 98 Xinghai South Street, Xingcheng 125100, China. Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Horticulture, Agricultural College of Shihezi University, Shihezi 832003, China.
- Key Laboratory of Horticulture Crops Germplasm Resources Utilization, Research Institute of Pomology, Chinese Academy of Agricultural Sciences (CAAS), Ministry of Agriculture and Rural Affairs of the People's Republic of China, No. 98 Xinghai South Street, Xingcheng 125100, China.
- Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Horticulture, Agricultural College of Shihezi University, Shihezi 832003, China.
Abstract
Apples are a major horticultural crop worldwide. Grafting techniques are widely utilized in apple production to keep the varieties pure. Interstocks are frequently used in Northern China to achieve intensive apple dwarfing cultivation. High-throughput sequencing was used to investigate differentially expressed genes in the phloem tissues of two different xenograft systems, M ('Gala'/'Mac 9'/ (L.) Borkh.) and B (/ (L.) Borkh.). The results showed that dwarfing interstocks could significantly reduce the height and diameters of apple trees while have few effects on the growth of annual branches. The interstocks were found to regulate the expression of genes related to hormone metabolism and tree body control (, , , , and ), these effects may attribute the dwarf characters for apple trees with interstocks. Besides, the interstocks reduce photosynthesis-related genes ( and ), promote carbon (C) metabolism gene expression (, and ), promote the expression of nitrogen (N)-metabolism-related genes (, and ) in rootstocks, and improve the expression of genes related to secondary metabolism in scions (, , and ). We also concluded that the interstocks acquired early blooming traits due to promotion of the expression of flowering genes in the scion (, , and ). This study is a valuable resource regarding the molecular mechanisms of dwarf interstocks' influence on various biological processes and transplantation systems in both scions and rootstocks.
MeSH
Genes, Gene Expression Profiling, Transcriptome