Journal Article Research Support, Non-U.S. Gov't Epublish 6 citations
PubMed: 36674321 DOI: 10.3390/ijerph20021564
Circulating Peptidome Is Strongly Altered in COVID-19 Patients.
Int J Environ Res Public Health 20(2)
Authors: Gianluca Baldanzi, Beatrice Purghè, Beatrice Ragnoli, Pier Paolo Sainaghi, Roberta Rolla, Annalisa Chiocchetti, Marcello Manfredi, Mario Malerba
Affiliations
- Department of Translational Medicine, University of Piemonte Orientale, 28100 Novara, Italy.
- Center for Translational Research on Autoimmune and Allergic Diseases, University of Piemonte Orientale, 28100 Novara, Italy.
- Respiratory Unit, Sant'Andrea Hospital, 13100 Vercelli, Italy.
- Internal and Emergency Medicine Department, Department of Translational Medicine, University of Piemonte Orientale, 28100 Novara, Italy.
- Department of Health Sciences, University of Piemonte Orientale, 28100 Novara, Italy.
Abstract
Whilst the impact of coronavirus disease 2019 (COVID-19) on the host proteome, metabolome, and lipidome has been largely investigated in different bio-fluids, to date, the circulating peptidome remains unexplored. Thus, the present study aimed to apply an untargeted peptidomic approach to provide insight into alterations of circulating peptides in the development and severity of SARS-CoV-2 infection. The circulating peptidome from COVID-19 severe and mildly symptomatic patients and negative controls was characterized using LC-MS/MS analysis for identification and quantification purposes. Database search and statistical analysis allowed a complete characterization of the plasma peptidome and the detection of the most significant modulated peptides that were impacted by the infection. Our results highlighted not only that peptide abundance inversely correlates with disease severity, but also the involvement of biomolecules belonging to inflammatory, immune-response, and coagulation proteins/processes. Moreover, our data suggested a possible involvement of changes in protein degradation patterns. In the present research, for the first time, the untargeted peptidomic approach enabled the identification of circulating peptides potentially playing a crucial role in the progression of COVID-19.
MeSH
Infections, Environmental Exposure, Covid-19