Journal Article Epublish 4 citations
PubMed: 36769239 DOI: 10.3390/ijms24032922
Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis.
Int J Mol Sci 24(3)
Authors: Sandra Sánchez-Jaut, Susana Pérez-Benavente, Paloma Abad, Darío Méndez-Cuadro, Antonio Puyet, Amalia Diez, Gonzalo Galicia-Poblet, Elena Gómez-Domínguez, María J Moran-Jiménez, José M Bautista, Isabel G Azcárate
Affiliations
- Department of Biochemistry and Molecular Biology, Complutense University of Madrid, 28040 Madrid, Spain.
- Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain.
- Analytical Chemistry and Biomedicine Group, School of Exact and Natural Sciences, San Pablo Campus, University of Cartagena, Cartagena 130014, Colombia.
- Pediatric Digestive Service, Guadalajara University Hospital, 19002 Guadalajara, Spain.
- Department of Medical Specialties and Public Health, Rey Juan Carlos University, 28922 Madrid, Spain.
Abstract
Iron overload caused by hereditary hemochromatosis (HH) increases free reactive oxygen species that, in turn, induce lipid peroxidation. Its 4-hydroxynonenal (HNE) by-product is a well-established marker of lipid peroxidation since it reacts with accessible proteins with deleterious consequences. Indeed, elevated levels of HNE are often detected in a wide variety of human diseases related to oxidative stress. Here, we evaluated HNE-modified proteins in the membrane of erythrocytes from HH patients and in organs of Hfe male and female mice, a mouse model of HH. For this purpose, we used one- and two-dimensional gel electrophoresis, immunoblotting and MALDI-TOF/TOF analysis. We identified cytoskeletal membrane proteins and membrane receptors of erythrocytes bound to HNE exclusively in HH patients. Furthermore, kidney and brain of Hfe mice contained more HNE-adducted protein than healthy controls. Our results identified main HNE-modified proteins suggesting that HH favours preferred protein targets for oxidation by HNE.
MeSH
Erythrocytes, Hemochromatosis, Oxidative Stress, Membranes