Journal Article Ppublish 1 citation
PubMed: 36803150 DOI: 10.1177/15910199231157926
A novel histological occlusion classification for coiled aneurysms based on multiphoton microscopy.
Interv Neuroradiol 31(3):324-332
Authors: Szatmary Zoltan, Cortese Jonathan, Mounier Jeremy, Perrin Marie-Laure, Janot Kevin, Couquet Claude, Aurélien Le Flahec, Leger-Bretou Claire, Mounayer Charbel, Rouchaud Aymeric, Sylvia M Bardet
Affiliations
- Neuroradiology Department, Limoges University, Dupuytren Hospital, Limoges, France. XLIM UMR CNRS 7252 Limoges, Aquitaine, France.
- XLIM UMR CNRS 7252 Limoges, Aquitaine, France. Neuroradiology Department, HĂ´pital BicĂªtre Interventional, Le Kremlin BicĂªtre, Ile-de-France, France.
- XLIM UMR CNRS 7252 Limoges, Aquitaine, France. Medical Faculty, Limoges University, Limoges, France.
- Medical Faculty, Limoges University, Limoges, France.
- XLIM UMR CNRS 7252 Limoges, Aquitaine, France. Neuroradiology Department, University Hospital of Tours, Tours, France.
- Neuroradiology Department, Limoges University, Dupuytren Hospital, Limoges, France.
- XLIM UMR CNRS 7252 Limoges, Aquitaine, France.
Abstract
ObjectiveIntracranial aneurysm (IA) coiling remains the most commonly used endovascular approach for ruptured and unruptured IA, and recanalization is a common drawback that impairs treatment success. Angiographic occlusion and aneurysm healing are not synonymous, and histological evaluation of embolized aneurysms remains a challenge. We propose here an experimental study of coil embolization in animal models by multiphoton microscopy (MPM) in comparison with conventional histological staining. The purpose of his work is to analyze coil healing process using histological sections of aneurysms.MethodsBased on a rabbit elastase model, 27 aneurysms were fixed, embedded in resin, and cut in thin histological sections 1 month after coils implantation and after angiographic control. Hematoxylin and eosin (H&S) staining were realized. Non-stained adjacent slices were imaged for multiphoton excited autofluorescence (AF) and second-harmonic generation (SHG) to construct three-dimensional (3D) projections of sequentially and axially acquired images.ResultsThe contrast provided by the combination of these two imaging modalities can be used to distinguish five levels of aneurysm healing, based on a combination of thrombus evolution and increased extracellular matrix (ECM) deposit.ConclusionRDPC:\Users\SHAHUL\RDP6|We have established a novel histological scale from a rabbit elastase aneurysm model after coiling with a classification of five different stages thanks to nonlinear microscopy. This classification is an actualized tool in order to obtain a more precise evaluation of occlusion device efficacy in the scope of new innovative microscopy for research.
MeSH
Neuroradiography, Microscopy, Histology, Aneurysm, Radiography, Interventional, Classification, Pancreatic Elastase