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Journal Article Epublish 8 citations

PubMed: 36911039 DOI: 10.3389/fped.2023.1133258

Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency.

Front Pediatr 11:1133258

Authors: Congfu Huang, Dongming Meng, Yinhu Li, Shiyang Lu, Wei Yang, Bin Wu, Shufen Chen, Zhenyu Yang, Haiying Liu

Affiliations

  • Department of Pediatrics, Longgang Maternity and Child Institute of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen, China.
  • Department of Computer Science, City University of Hong Kong, Hong Kong, Hong Kong SAR, China.
  • Department of Pediatrics, The People's Hospital of Shenzhen Baoan District, Shenzhen, China.
  • School of Statistics and Data Science, NanKai University, Tianjin, China.
  • Department of Pediatrics, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, China.

Abstract

Objective

To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.

Methods

Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group. Total DNA was extracted and amplified from stool samples obtained from all subjects. High-throughput sequencing was used to analyze the GM composition and functions.

Results

The GM from the two groups of children showed significant differences in α-diversity ( < 0.05). In comparison with the control group, the abundance of the phylum was significantly higher (45.96% vs. 65.71%) while the count was significantly lower (47.09% vs. 25.20%). At the genus level, the abundance of in the disease group was significantly higher (3.16% vs. 20.67%) and that of , XlVa, and was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively). GM functions, including those involved in membrane_transport, energy_metabolism, poorly_characterized, metabolism_of_cofactors_and_vitamins, glycan_biosynthesis_and_metabolism, transcription, folding,_sorting,_and_degradation, were significantly altered in the disease group. The abundance of various GM components was correlated with endocrine hormone levels.

Conclusion

Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.

MeSH

Child, Microbiota, Hormones, Growth Hormone, Gastrointestinal Microbiome

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