Journal Article Ppublish 6 citations
PubMed: 36969951 DOI: 10.1016/j.arabjc.2023.104813
Bio-guided chemical characterization and nano-formulation studies of selected edible volatile oils with potentials antibacterial and anti-SARS-CoV-2 activities.
Arab J Chem 16(7):104813
Authors: Mohamed S Refaey, Marwa A A Fayed, Omnia Kutkat, Yassmin Moatasim, Nahla Sameh Tolba, Anis Anis, Ahmed M Elshorbagy, Khloud Nassar, Khaled A M Abouzid, Yaseen A M M Elshaier, Mohamed F El-Badawy
Affiliations
- Department of Pharmacognosy, Faculty of Pharmacy, University of Sadat City, Menoufia 32897, Egypt.
- Center of Scientific Excellence for Influenza Viruses, National Research Centre (NRC), Giza 12622, Egypt.
- Department of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, Menoufia 32897, Egypt.
- Department of Pathology, faculty of Veterinary Medicine, University of Sadat City, Egypt.
- Department of biochemistry, Faculty of Pharmacy, University of Sadat City, Menoufia 32897, Egypt.
- Pharmaceutical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Abbassia 11566, Cairo, Egypt.
- Department of Organic and Medicinal Chemistry, Faculty of Pharmacy, University of Sadat City, Menoufia 32897, Egypt.
- Microbiology and immunology Department, Faculty of Pharmacy, University of Sadat City, Menoufia 32897, Egypt.
Abstract
The recent pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has opened the door to potential threats of the respiratory system. The discovery of drugs from natural sources is one of the most important strategies for treating the upper respiratory tract. In this study, we investigated the selected formulated EOs activities against Gram-negative and ) and Gram-positive () bacteria and against the SARS-CoV-2 virus, with the mode of action investigated as anti-SARS-CoV-2. and EOs were the most promising antibacterial oils. EO showed MIC values of 1, 1, 2, ≤0.5, and 8 µg/mL against , and , respectively, while EO showed MIC values of 8, 4, 32, 8, 32 µg/mL against the same organisms. The cytotoxic activity of the oil samples was tested in VERO-E6 cells using (MTT) assay and showed that the safest oil was , then , , , and . The most potent antiviral EOs were oil and , with IC value of 15.16 and 96.5 µg/mL, respectively. Moreover, the safety index of EO (26.3) was greater than the oil of (7.25). The mechanism by which oil exerts its antiviral activity may involve both the virucidal effect and its impact on viral reproduction. The nano-emulsion dosage form of the potent EOs was prepared and re-examined against the same bacterial and viral strains. Finally, the chemical characterization of these promising essential oils was analyzed and identified using the GC-MS approach. To the best of our knowledge, this is the first report concerning the investigation of anti-SARS-CoV-2 activity of these selected essential oils, along with a proposed mechanism for the potent oil's activity.
MeSH
Gram-Positive Bacteria, Oils, Volatile, Sars Virus, Sars-Cov-2