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Publications

Journal Article Epublish 1 citation

PubMed: 37057238 DOI: 10.4103/abr.abr_24_22

Toll-Like Receptor 7 a Novel Non-Invasive Inflammatory Genetic Sensor for Ulcerative Colitis Remission Monitoring.

Adv Biomed Res 12:54

Authors: Hamid Asadzadeh-Aghdaei, Leili Rejali, Mahyar Nourian, Vahid Chaleshi, Naghmeh Zamani, Shaghayegh Baradaran-Ghavami, Mohsen Nemati, Shabnam Shahrokh, Mohsen Norouzinia, Massoud Vosough, Ehsan Nazemalhosseini-Mojarad, Mohammadreza Zali

Affiliations

  • Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Mahak Hematology Oncology Research Center (Mahak-HORC), Mahak Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.

Abstract

Background

Ulcerative colitis (UC) and Crohn's disease (CD) are two major types of inflammatory bowel diseases (IBDs). Toll-like receptors (TLRs) are expressed in the innate immune system compartments, in charge of identifying a wide range of microorganisms. The aim of the present study was to evaluate the expression of , -, and - in peripheral blood mononuclear cells (PBMC) of UC patients as a novel non-invasive primary inflammation sensor for monitoring the clinical course of UC candidates.

Materials and Methods

In this cross-sectional study, total RNA was extracted from the PBMC of 42 UC patients along with 20 healthy donors. The mRNA levels of , -, and - were assessed using the quantitative real-time polymerase chain (qRT-PCR) reaction.

Results

The present research study demonstrated no significant changes in mRNA expression in UC patients in comparison with the control group ( = 0.1264), whereas significant elevation ( = 0.0008) was distinguished in the expression of UC participants specifically during the remission course compared with healthy donors and flareup patients ( = 0.0004 and = 0.0063, respectively). The last selected TLR, was not shown remarkable changes either between UC patients and the control group or between clinical courses of the disease.

Conclusion

Here, among three nominated TLRs for predicting UC patients, -7 was potentially selected according to the significant difference in mRNA expression in flareup UC patients and control donors. -7 could be used as a novel non-invasive biomarker for monitoring UC patients in the active course of the disease.

MeSH

Rna, Inflammatory Bowel Diseases, Biomarkers, Colitis, Ulcerative, Toll-Like Receptors, Toll-Like Receptor 7