System Maintenance Notice

Please note that maintenance work is scheduled between 4 AM and 5 AM - CET. During this time, you may experience reduced performance. Thanks for your patience.

Publications

Journal Article Ppublish 1 citation

PubMed: 37166640 DOI: 10.1007/978-1-0716-3171-3_13

Assembly of the Mitochondrial Translation Initiation Complex.

Methods Mol Biol 2661:217-232

Authors: Cristina Remes, Minh Duc Nguyen, Henrik Spahr, Martin Ng, Clark Fritsch, Arpan Bhattacharya, Hong Li, Barry Cooperman, Joanna Rorbach

Affiliations

  • Department of Chemistry, University of Pennsylvania, Philadelphia, PA, USA.
  • Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solna, Sweden.
  • Department of Chemistry, University of Pennsylvania, Philadelphia, PA, USA.
  • Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Max Planck Institute Biology of Ageing, Karolinska Institutet Laboratory, Karolinska Institutet, Stockholm, Sweden.

Abstract

Mitochondria maintain their own translational machinery that is responsible for the synthesis of essential components of the oxidative phosphorylation system. The mammalian mitochondrial translation system differs significantly from its cytosolic and bacterial counterparts. Here, we describe detailed protocols for efficient in vitro reconstitution of the mammalian mitochondrial translation initiation complex, which can be further used for mechanistic analyses of different aspects of mitochondrial translation.

MeSH

Mitochondria, Mitochondrial Ribosomes

Cited by