Journal Article Review Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Epublish 8 citations
PubMed: 37296674 DOI: 10.3390/cells12111554
Generation of Red Blood Cells from Human Pluripotent Stem Cells-An Update.
Cells 12(11)
Authors: Shin-Jeong Lee, Cholomi Jung, Jee Eun Oh, Sangsung Kim, Sangho Lee, Ji Yoon Lee, Young-Sup Yoon
Affiliations
- Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. Research and Development Center, KarisBio Inc. 50-1 Yonsei-Ro, Avison Biomedical Research Center Room 525, Seodaemun-gu, Seoul 03722, Republic of Korea.
- Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
- Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.
- Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
- Research and Development Center, KarisBio Inc. 50-1 Yonsei-Ro, Avison Biomedical Research Center Room 525, Seodaemun-gu, Seoul 03722, Republic of Korea.
Abstract
Red blood cell (RBC) transfusion is a lifesaving medical procedure that can treat patients with anemia and hemoglobin disorders. However, the shortage of blood supply and risks of transfusion-transmitted infection and immune incompatibility present a challenge for transfusion. The in vitro generation of RBCs or erythrocytes holds great promise for transfusion medicine and novel cell-based therapies. While hematopoietic stem cells and progenitors derived from peripheral blood, cord blood, and bone marrow can give rise to erythrocytes, the use of human pluripotent stem cells (hPSCs) has also provided an important opportunity to obtain erythrocytes. These hPSCs include both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). As hESCs carry ethical and political controversies, hiPSCs can be a more universal source for RBC generation. In this review, we first discuss the key concepts and mechanisms of erythropoiesis. Thereafter, we summarize different methodologies to differentiate hPSCs into erythrocytes with an emphasis on the key features of human definitive erythroid lineage cells. Finally, we address the current limitations and future directions of clinical applications using hiPSC-derived erythrocytes.
MeSH
Infections, Erythrocytes, Blood Cells, Microscopy, Electron, Scanning Transmission, Erythroid Cells, Hematopoietic Stem Cells, Pluripotent Stem Cells, Human Embryonic Stem Cells, Transfusion Reaction