Journal Article Ppublish 17 citations
PubMed: 37703362 DOI: 10.1126/sciadv.adg6231
Noninvasive morpho-molecular imaging reveals early therapy-induced senescence in human cancer cells.
Sci Adv 9(37):eadg6231
Authors: Arianna Bresci, Jeong Hee Kim, Silvia Ghislanzoni, Francesco Manetti, Lintong Wu, Federico Vernuccio, Chiara Ceconello, Salvatore Sorrentino, Ishan Barman, Italia Bongarzone, Giulio Cerullo, Renzo Vanna, Dario Polli
Affiliations
- Department of Physics, Politecnico di Milano, Milan, Italy.
- Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
- Department of Advanced Diagnostics, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy.
- Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
- The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
- CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), Milan, Italy.
Abstract
Anticancer therapy screening in vitro identifies additional treatments and improves clinical outcomes. Systematically, although most tested cells respond to cues with apoptosis, an appreciable portion enters a senescent state, a critical condition potentially driving tumor resistance and relapse. Conventional screening protocols would strongly benefit from prompt identification and monitoring of therapy-induced senescent (TIS) cells in their native form. We combined complementary all-optical, label-free, and quantitative microscopy techniques, based on coherent Raman scattering, multiphoton absorption, and interferometry, to explore the early onset and progression of this phenotype, which has been understudied in unperturbed conditions. We identified TIS manifestations as early as 24 hours following treatment, consisting of substantial mitochondrial rearrangement and increase of volume and dry mass, followed by accumulation of lipid vesicles starting at 72 hours. This work holds the potential to affect anticancer treatment research, by offering a label-free, rapid, and accurate method to identify initial TIS in tumor cells.
MeSH
Cells, Biological Science Disciplines, Neoplasms, Therapeutics, Molecular Imaging
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