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Journal Article Epublish 7 citations

PubMed: 37851174 DOI: 10.1007/s00018-023-04986-3

Molecular tracking of interactions between progenitor and endothelial cells via Raman and FTIR spectroscopy imaging: a proof of concept of a new analytical strategy for in vitro research.

Cell Mol Life Sci 80(11):329

Authors: Karolina Augustyniak, Aleksandra Pragnaca, Monika Lesniak, Marta Halasa, Agata Borkowska, Ewa Pieta, Wojciech M Kwiatek, Claudine Kieda, Robert Zdanowski, Kamilla Malek

Affiliations

  • Department of Chemical Physics, Faculty of Chemistry, Jagiellonian University in Krakow, Gronostajowa 2, 30-387, Krakow, Poland.
  • Doctoral School of Exact and Natural Sciences, Jagiellonian University in Krakow, Prof. S. Lojasiewicza 11, 30-348, Krakow, Poland.
  • Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine-National Research Institute, Szaserow 128, 04-141, Warsaw, Poland.
  • Transplant Immunology, The Houston Methodist Research Institute, Houston, TX, USA.
  • Department of Surgery, The Houston Methodist Hospital, Houston, TX, USA.
  • Postgraduate School of Molecular Medicine, Medical University of Warsaw, Zwirki i Wigury 61, 02-091, Warsaw, Poland.
  • Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342, Krakow, Poland.
  • Center for Molecular Biophysics, UPR4301 CNRS, Orleans, France.
  • Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine-National Research Institute, Szaserow 128, 04-141, Warsaw, Poland.
  • Department of Chemical Physics, Faculty of Chemistry, Jagiellonian University in Krakow, Gronostajowa 2, 30-387, Krakow, Poland.

Abstract

Circulating endothelial cell progenitors originating from the bone marrow are considered to be a powerful tool in the repair of endothelium damage. Due to their unique properties, endothelial progenitors are now broadly investigated to assess their clinical significance in diseases e.g., associated with brain endothelial dysfunction. However, their distinction in terms of the expression of specific markers remains ambiguous. Additionally, endothelial progenitor cells may change their repertoire of markers depending on the microenvironment of the tissue in which they are currently located. Here, we applied the label-free Raman and FTIR imaging to discriminate mice brain endothelium and endothelial progenitors. Cells cultured separately showed distinctly different spectral signatures extracted from the whole cellular interior as well as the detected intracellular compartments (nucleus, cytoplasm, perinuclear area, and lipid droplets). Then, we used these spectroscopic signals to examine the cells co-cultured for 24 h. Principal cluster analysis showed their grouping with the progenitor cells and segregation from brain endothelium at a level of the entire cell machinery (in FTIR images) which resulted from biochemical alternations in the cytoplasm and lipid droplets (in Raman images). The models included in partial least square regression indicated that lipid droplets are the key element for the classification of endothelial progenitor-brain endothelial cells interactions.

MeSH

Cells, Lipid Droplets, Biological Science Disciplines, Brain, Stem Cells, Cell Biology, Cytoplasm, Endothelial Cells, Research, Endothelium

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