Journal Article Research Support, Non-U.S. Gov't Epublish
PubMed: 37883361 DOI: 10.1371/journal.pone.0293356
New erythrocyte parameters derived from the Coulter principle relate with red blood cell properties-A pilot study in diabetes mellitus.
PLoS One 18(10):e0293356
Authors: Chloé Bourguignon, Clémentine Ansel, Jean-Philippe Gineys, Sophie Schuldiner, Damien Isèbe, Michael Geitner, Pierre Taraconat, Jean-Christophe Gris
Affiliations
- Department of Haematology, Nîmes University Hospital and University of Montpellier, Nîmes, France.
- UMR UA11 INSERM - Montpellier University IDESP, Montpellier, France.
- Department of Haematology, Nîmes University Hospital and University of Montpellier, Nîmes, France. HORIBA Medical, Parc Euromédecine, Rue du Caducée, Montpellier, France.
- HORIBA Medical, Parc Euromédecine, Rue du Caducée, Montpellier, France.
- Department of Metabolic Diseases and Endocrinology, Nîmes University Hospital, Nîmes, France.
- Department of Gynaecology and Obstetrics, First Moscow State Medical University (Sechenov University), Moscow, Russian Federation.
Abstract
In routine hematological instruments, blood cells are counted and sized by monitoring the impedance signals induced during their passage through a Coulter orifice. However, only signals associated with centered paths in the aperture are considered for analysis, while the rejected measurements, caused by near-wall trajectories, can provide additional information on red blood cells (RBC), as recent publications suggest. To assess usefulness of two new parameters in describing alterations in RBC properties, we performed a pilot study to compare blood samples from patients with diabetes mellitus (DM), frequent pathological condition associated with impairment in RBC deformability, versus controls. A total of 345 blood samples were analyzed: 225 in the DM group and 120 in the control group. A diagram of [Formula: see text] and [Formula: see text], the two new parameters derived from the analysis of impedancemetry pulses, was used to compare distribution of RBC subpopulations between groups. To discriminate RBC from DM and control individuals, based on our multiparametric analysis, we built a score from variables derived from [Formula: see text] matrix which showed good performances: area under the receiving operating characteristic curve 0.948 (0.920-0.970), p<0.0001; best discriminating value: negative predictive value 94.7%, positive predictive value was 78.4%. These results seem promising to approach RBC alterations in routine laboratory practice. The related potential clinically relevant outcomes remain to be investigated.
MeSH
Erythrocytes, Pilot Projects, Blood Cells, Diabetes Mellitus, Pilots