Abstract

Tuberculosis is a major infectious disease caused by infection. The pathogenesis and immune mechanism of tuberculosis are not clear, and it is urgent to find new drugs, diagnosis, and treatment targets. A useful tool in the quest to reveal the enigmas related to infection and disease is the single-cell sequencing technique. By clarifying cell heterogeneity, identifying pathogenic cell groups, and finding key gene targets, the map at the single cell level enables people to better understand the cell diversity of complex organisms and the immune state of hosts during infection. Here, we briefly reviewed the development of single-cell sequencing, and emphasized the different applications and limitations of various technologies. Single-cell sequencing has been widely used in the study of the pathogenesis and immune response of tuberculosis. We review these works summarizing the most influential findings. Combined with the multi-molecular level and multi-dimensional analysis, we aim to deeply understand the blank and potential future development of the research on infection using single-cell sequencing technology.

MeSH

Infections, Immune System Diseases, Tuberculosis, Cells, Genes, Research, Host-Pathogen Interactions

Cited by

• Host Circulating Immunometabolism-Associated Biomarkers for Early Diagnosis of Active Tuberculosis: Multi-Omics Screening with Experimental Validation. J Inflamm Res, 2025
• Experimental dissection of tuberculosis protective immunity: a human perspective. Front Cell Infect Microbiol, 2025
• Single-cell sequencing: Current applications in various tuberculosis specimen types. Cell Prolif, 2024