Journal Article Research Support, Non-U.S. Gov't Epublish 5 citations
PubMed: 37903839 DOI: 10.1038/s41598-023-45575-5
Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers.
Sci Rep 13(1):18570
Authors: Dominik Drabik, Mitja Drab, Samo Penič, Aleš Iglič, Aleksander Czogalla
Affiliations
- Laboratory of Cytobiochemistry, Faculty of Biotechnology, University of Wroclaw, F. Joliot-Curie 14a, 50-383, Wrocław, Poland.
- Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Pl. Grunwaldzki 13, 50-377, Wrocław, Poland.
- Laboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška cesta 25, 1000, Ljubljana, Slovenia.
- Laboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška cesta 25, 1000, Ljubljana, Slovenia.
- Laboratory of Bioelectromagnetics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška cesta 25, 1000, Ljubljana, Slovenia.
- Laboratory of Cytobiochemistry, Faculty of Biotechnology, University of Wroclaw, F. Joliot-Curie 14a, 50-383, Wrocław, Poland.
Abstract
Biological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techniques required for quantitative investigation present significant challenges. In this study we employ spot-variation z-scan fluorescence correlation spectroscopy (svzFCS) tailored for artificial lipid vesicles of varying composition and combine this approach with high-resolution imaging. This method has been harnessed to examine the lipid-segregation behavior of distinct types of ceramide-1-phosphate (C1P), a crucial class of signaling molecules, within these membranes. Moreover, we provide a quantitative portrayal of the lipid membranes studied and the domains induced by C1P at both nano and microscales. Given the lack of definitive conclusions from the experimental data obtained, it was supplemented with comprehensive in silico studies-including the analysis of diffusion coefficient via molecular dynamics and domain populations via Monte Carlo simulations. This approach enhanced our insight into the dynamic behavior of these molecules within model lipid membranes, confirming that nano- and microdomains can co-exist in lipid vesicles.
MeSH
Ceramides, Natural Science Disciplines, Lipid Bilayers, Membranes