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Journal Article Epublish 2 citations

PubMed: 37963988 DOI: 10.1038/s41598-023-47170-0

Optimization of oil yield of Pelargonium graveolens L'Hér using Box-Behnken design in relation to its antimicrobial activity and in silico study.

Sci Rep 13(1):19887

Authors: Sanagik Sabry Abu El Wafa, Ahmed A El-Ashmawy, Hanaa A H Kassem, Ibrahim H Eissa, Mohammed Abu-Elghait, Nermin A Younis, Inas Y Younis

Affiliations

  • Pharmacognosy Department, Faculty of Pharmacy, Ahram Canadian University, Giza, 12451, Egypt.
  • Medicinal and Pharmaceutical Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Giza, 12622, Egypt.
  • Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.
  • Pharmaceutical Medicinal Chemistry and Drug Design Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, 11884, Egypt.
  • Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, Egypt.
  • Pharmacognosy Department, Faculty of Pharmacy, Ahram Canadian University, Giza, 12451, Egypt.
  • Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.

Abstract

Pelargonium graveolens L'Hér is an important species of genus Pelargonium with an economic value. The unique rose scent of its oil is used in perfume and cosmetic industry. The oil is characterized by the presence of citronellol, geraniol and rose oxide. Fresh aerial parts of P. graveolens at GC-MS analysis of four seasons revealed that autumn constituted the highest yield of the oil. For the first time, optimization of the yield of extracted oil of P. graveolens was performed employing 3-level Box-Behnken design using 3-factors. The GC-MS analysis of the essential oil was performed for the 17-runs. The optimized extraction of the oil was performed employing numerical optimization and studied for antimicrobial, Minimum Inhibitory Concentration (MIC) and biofilm inhibitory activities. The 3 factors followed rank (plant material amount > water volume > NaCl percent in water), in their magnitude of effect on increasing yield of the oil. Increasing the plant material amount increased the yield of the oil by 6-folds compared to NaCl percent in water. The optimized yield of oil (4 ml) was obtained from extraction criteria (150 g of plant, 750 ml of water and 3.585% (26.85 g) of NaCl). Computational docking was performed to overcome the multi-drug resistant Gram-negative bacilli targeting undecaprenyl pyrophosphate synthase (UPPS). The optimized oil exhibited a promising inhibitory activity against Gram-negative bacteria (K. pneumonia and P. aeruginosa) with significant antibiofilm action (P < 0.05). Moreover, it exerted a synergistic effect when combined with various antibiotics (Cefoxitin, Cloxacillin, Oxacillin and Vancomycin) against MRSA clinical strains.

MeSH

Natural Science Disciplines, Biofilms, Gram-Negative Bacteria