Journal Article Epublish 12 citations
PubMed: 38026528 DOI: 10.2147/IJN.S428924
Emodin Ameliorates Severe Acute Pancreatitis-Associated Acute Lung Injury in Rats by Modulating Exosome-Specific miRNA Expression Profiles.
Int J Nanomedicine 18:6743-6761
Authors: Qi Yang, Yalan Luo, Peng Ge, Bowen Lan, Jin Liu, Haiyun Wen, Yinan Cao, Zhenxuan Sun, Guixin Zhang, Huiming Yuan, Lihua Zhang, Hailong Chen
Affiliations
- Department of General Surgery, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116011, People's Republic of China. Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, 116011, People's Republic of China. Laboratory of Integrative Medicine, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116011, People's Republic of China. Department of Traditional Chinese Medicine, the Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, People's Republic of China.
- Department of General Surgery, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116011, People's Republic of China. Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, 116011, People's Republic of China. Laboratory of Integrative Medicine, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116011, People's Republic of China.
- Department of General Surgery, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116011, People's Republic of China.
- Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, 116011, People's Republic of China.
- Laboratory of Integrative Medicine, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116011, People's Republic of China.
- CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, People's Republic of China.
Abstract
Background
Numerous preclinical investigations have exhibited the beneficial impact of emodin (EMO) on the management of severe acute pancreatitis (SAP)-associated acute lung injury (ALI). However, the potential of EMO to mitigate organ damage through the modulation of exosome (Exo)-specific miRNA expression profiles remains unclear.
Methods
The SAP rat model was established by retrograde injection of 5% sodium taurocholate into the pancreatic bile duct. Rats received intragastric administration of EMO at 2 h and 12 h post-modeling. Plasma and bronchoalveolar lavage fluid (BALF)-derived exosomes were isolated and purified from SAP rats treated with EMO. The therapeutic effects of these Exos in SAP rats were assessed using hematoxylin-eosin staining and measurement of inflammatory factor levels. MicroRNA (miRNA) sequencing was conducted on plasma and BALF-derived Exos, and rescue experiments were performed to investigate the function of NOVEL miR-29a-3p in the treatment of SAP using EMO.
Results
EMO exhibits ameliorative effects on pancreatic and lung injury and inflammation in rats with SAP. Plasma/BALF-derived Exos from EMO-treated SAP rats also have therapeutic effects on SAP rats. The miRNA expression profile of plasma and BALF-derived Exos in SAP rats underwent significant changes upon exposure to EMO. In particular, 34 differentially expressed miRNAs (DEmiRNAs) were identified when comparing BALF-SAP+EMO-Exo and BALF-SAP-Exo. 39 DEmiRNAs were identified when comparing plasma-SAP+EMO-Exo to plasma-SAP-Exo. We found that SAP rats treated with Exos derived from BALF exhibited a more potent therapeutic response than those treated with Exos derived from plasma. EMO may rely on NOVEL-rno-miR-29a-3p expression to prevent pulmonary injury in SAP rats.
Conclusion
The mechanism of action of EMO is observed to have a significant impact on the miRNA expression profile of Exos derived from plasma and BALF in SAP rats. NOVEL-rno-miR-29a-3p, which is specific to Exos, and is derived from BALF, may play a crucial role in the therapeutic efficacy of EMO.
MeSH
Pancreatitis, Plasma, Therapeutics, Exosomes, Acute Lung Injury, Nanomedicine, Emodin, Respiratory Distress Syndrome, Exhibitions As Topic, Micrornas
Cited by
- Emodin protects against severe acute pancreatitis-associated acute lung injury by activating Nrf2/HO-1/GPX4 signal and inhibiting ferroptosis in vivo and in vitro. BMC Gastroenterol, 2025
- Emodin: an alveolar macrophage protector in acute pancreatitis induced lung injury. Int J Med Sci, 2025
- Dachengqi decoction for the treatment of acute pancreatitis: a comprehensive analysis based on metabolites, pharmacokinetics, and metabolites efficacy mechanisms. Front Pharmacol, 2025
- Exosome-like nanoparticles derived from fruits, vegetables, and herbs: innovative strategies of therapeutic and drug delivery. Theranostics, 2024
- Multi-Target Synergy Against Acute Lung Injury: Systems Pharmacology Decoding Dahuang-Huangqin Herb Pair's Therapeutic Mechanism. J Inflamm Res, 2025
- Epigenetic regulatory mechanism of macrophage polarization in diabetic wound healing (Review). Mol Med Rep, 2024
- Exosomal miRNAs in pancreatitis: Mechanisms and potential applications (Review). Mol Med Rep, 2025