Journal Article Epublish 1 citation
PubMed: 38035022 DOI: 10.3389/fphar.2023.1235190
protects against metal induced-toxicity in .
Front Pharmacol 14:1235190
Authors: Margareth Duran-Izquierdo, Lucellys Sierra-Marquez, Maria Taboada-Alquerque, Jesus Olivero-Verbel
Affiliations
- Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, University of Cartagena, Zaragocilla Campus, Cartagena, Colombia.
Abstract
(Hook.f.) Steyerm (Rubiaceae) is a vascular plant used in Northern Colombia as a source of pigments and wood. However, there is a lack of information regarding its pharmacology and toxicity. This research aimed to study the hydroalcoholic extract of as a protector against metal-induced toxicity in . Preliminary phytochemical screening of the hydroalcoholic extract of (HAE-Sc) was conducted using HPLC-ESI-QTOF. Wild-type N2 larvae were exposed to different concentrations of HAE-Sc evaluating lethality (50-5000 μg/mL), growth, lifespan, resistance to heat stress, and its protective effect against Mercury (Hg)-, Lead (Pb)- and Cadmium (Cd)-induced lethality (50-1000 μg/mL). The main metabolites present in the extract were iridoids, β-carboline-alkaloids and polyphenols. Bioassays demonstrated that HAE-Sc exhibited low toxicity, with significant lethality (4.2% and 9.4%) occurring at 2500-5000 μg/mL. Growth inhibition reached up to 23.3%, while reproduction declined 13% and 17% at concentrations 500 and 1000 μg/mL, respectively. HAE-Sc enhanced the survival rate of the nematode under thermal stress by up to 79.8%, and extended the mean lifespan of worms by over 33% compared to control. The average lifespan was prolonged by 15.3% and 18.5% at 50 and 100 μg/mL HAE-Sc, respectively. The extract (1000 μg/mL) was able to reduce the death of in the presence of heavy metals up to 65.9, 96.8% and 87% for Pb, Hg, and Cd, respectively. In summary, shows potential protective effects in against toxicity caused by heavy metals and heat.
MeSH
Alkaloids, Stress, Physiological, Biodiversity