Journal Article Epublish 18 citations
PubMed: 38216871 DOI: 10.1186/s12866-023-03171-7
Biogenic selenium nanoparticles and selenium/chitosan-Nanoconjugate biosynthesized by Streptomyces parvulus MAR4 with antimicrobial and anticancer potential.
BMC Microbiol 24(1):21
Authors: Mervat G Hassan, Mariam T Hawwa, Dina M Baraka, Hamed M El-Shora, Ahmed A Hamed
Affiliations
- Botany and Microbiology Department, Faculty of Science, Benha University, P. O. Box 13511, Banha, Qalyubia, Egypt.
- Botany Department, Faculty of Science, Mansoura University, P. O. Box 35516, Mansoura, Dakahliaو, Egypt.
- Microbial Chemistry Department, National Research Centre, 33 El-Buhouth Street, P. O. Box 12622, Giza, Dokki, Egypt.
Abstract
BACKGROUND
As antibiotics and chemotherapeutics are no longer as efficient as they once were, multidrug resistant (MDR) pathogens and cancer are presently considered as two of the most dangerous threats to human life. In this study, Selenium nanoparticles (SeNPs) biosynthesized by Streptomyces parvulus MAR4, nano-chitosan (NCh), and their nanoconjugate (Se/Ch-nanoconjugate) were suggested to be efficacious antimicrobial and anticancer agents.
RESULTS
SeNPs biosynthesized by Streptomyces parvulus MAR4 and NCh were successfully achieved and conjugated. The biosynthesized SeNPs were spherical with a mean diameter of 94.2 nm and high stability. Yet, Se/Ch-nanoconjugate was semispherical with a 74.9 nm mean diameter and much higher stability. The SeNPs, NCh, and Se/Ch-nanoconjugate showed significant antimicrobial activity against various microbial pathogens with strong inhibitory effect on their tested metabolic key enzymes [phosphoglucose isomerase (PGI), pyruvate dehydrogenase (PDH), glucose-6-phosphate dehydrogenase (G6PDH) and nitrate reductase (NR)]; Se/Ch-nanoconjugate was the most powerful agent. Furthermore, SeNPs revealed strong cytotoxicity against HepG2 (IC 50 = 13.04 μg/ml) and moderate toxicity against Caki-1 (HTB-46) tumor cell lines (IC 50 = 21.35 μg/ml) but low cytotoxicity against WI-38 normal cell line (IC 50 = 85.69 μg/ml). Nevertheless, Se/Ch-nanoconjugate displayed substantial cytotoxicity against HepG2 and Caki-1 (HTB-46) with IC 50 values of 11.82 and 7.83 μg/ml, respectively. Consequently, Se/Ch-nanoconjugate may be more easily absorbed by both tumor cell lines. However, it exhibited very low cytotoxicity on WI-38 with IC 50 of 153.3 μg/ml. Therefore, Se/Ch-nanoconjugate presented the most anticancer activity.
CONCLUSION
The biosynthesized SeNPs and Se/Ch-nanoconjugate are convincingly recommended to be used in biomedical applications as versatile and potent antimicrobial and anticancer agents ensuring notable levels of biosafety, environmental compatibility, and efficacy.
MeSH
Microbiology, Oxidoreductases, Cells, Neoplasms, Nanoparticles, Chitosan, Glucosephosphate Dehydrogenase, Antineoplastic Agents, Streptomyces, Glucose-6-Phosphate, Cell Line, Tumor, Nanoconjugates
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