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Journal Article Epublish 10 citations

PubMed: 38232064 DOI: 10.1371/journal.pone.0290938

Multilocus sequence typing of Candida albicans oral isolates reveals high genetic relatedness of mother-child dyads in early life.

PLoS One 19(1):e0290938

Authors: Naemah Alkhars, Nisreen Al Jallad, Tong Tong Wu, Jin Xiao

Affiliations

  • Department of General Dental Practice, College of Dentistry, Health Science Center, Kuwait University, Safat, Kuwait.
  • Translational Biomedical Science Program, Clinical and Translational Science Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
  • Eastman Institute for Oral Health, University of Rochester Medical Center, Rochester, New York, United States of America.
  • Department of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester, New York, United States of America.

Abstract

Candida albicans is a pathogenic fungus recently recognized for its role in severe early childhood caries development (S-ECC). C. albicans oral colonization begins at birth, but the extent of the mother's involvement in yeast transmission to their children is unclear, therefore, this study used a prospective mother-infant cohort to investigate the maternal contribution of C. albicans oral colonization in early life. Oral samples were collected from 160 mother-child dyads during pregnancy and from birth to two years of life. We used whole-genome sequencing to obtain the genetic information of C. albicans isolates and examined the genetic relatedness of C. albicans between mothers and their children using Multilocus Sequence Typing. Multivariate statistical methods were used to identify factors associated with C. albicans' acquisition (horizontal and vertical transmissions). Overall, 227 C. albicans oral isolates were obtained from 93 (58.1%) of mother-child pairs. eBURST analysis revealed 16 clonal complexes, and UPGMA analysis identified 6 clades, with clade 1 being the most populated 124 isolates (54.6%). Significantly, 94% of mothers and children with oral C. albicans had highly genetically related strains, highlighting a strong maternal influence on children's C. albicans acquisition. Although factors such as race, ethnicity, delivery method, and feeding behaviors did not show a significant association with C. albicans vertical transmission, the mother's oral hygiene status reflected by plaque index (PI) emerged as a significant factor; Mothers with higher dental plaque accumulation (PI >=2) had a significantly increased risk of vertically transmitting C. albicans to their infants [odds ratio (95% confidence interval) of 8.02 (1.21, 53.24), p=0.03]. Furthermore, Black infants and those who attended daycare had an elevated risk of acquiring C. albicans through horizontal transmission (p <0.01). These findings highlight the substantial role of maternal transmission in the oral acquisition of C. albicans during early life. Incorporating screening for maternal fungal oral carriage and implementing oral health education programs during the perinatal stage may prove valuable in preventing fungal transmission in early infancy.

MeSH

Multilocus Sequence Typing, Mothers, Parturition, Infectious Disease Transmission, Vertical, Candida Albicans, Risk, Child, Infant

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