Journal Article Epublish 6 citations
PubMed: 38326394 DOI: 10.1038/s41598-024-53658-0
A new bio imagery user-friendly tool for automatic morphometry measurement on muscle cell cultures and histological sections.
Sci Rep 14(1):3108
Authors: Aurélien Brun, Guillaume Mougeot, Philippe Denis, Marie Laure Collin, Pierre Pouchin, Christophe Montaurier, Stéphane Walrand, Frédéric Capel, Marine Gueugneau
Affiliations
- UMR1019 Unité de Nutrition Humaine (UNH), INRAE, Université Clermont Auvergne, Clermont-Ferrand, France.
- iGReD CNRS, INSERM Université Clermont Auvergne, Clermont-Ferrand, France.
- UMR1019 Unité de Nutrition Humaine (UNH), INRAE, Université Clermont Auvergne, Clermont-Ferrand, France.
Abstract
TRUEFAD (TRUE Fiber Atrophy Distinction) is a bioimagery user-friendly tool developed to allow consistent and automatic measurement of myotube diameter in vitro, muscle fiber size and type using rodents and human muscle biopsies. This TRUEFAD package was set up to standardize and dynamize muscle research via easy-to-obtain images run on an open-source plugin for FIJI. We showed here both the robustness and the performance of our pipelines to correctly segment muscle cells and fibers. We evaluated our pipeline on real experiment image sets and showed consistent reliability across images and conditions. TRUEFAD development makes possible systematical and rapid screening of substances impacting muscle morphology for helping scientists focus on their hypothesis rather than image analysis.
MeSH
Natural Science Disciplines, Research Design, Imagery, Psychotherapy, Muscle Cells, Muscles, Cell Culture Techniques