Journal Article Epublish 2 citations
PubMed: 38481883 DOI: 10.7759/cureus.54031
Anticancer Effects of Rosmarinus officinalis Leaf Extract on KB Cell Lines.
Cureus 16(2):e54031
Authors: V R Pradeep, S Menaka, Vasugi Suresh, Selvaraj Jayaraman
Affiliations
- Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, IND.
- Centre of Molecular Medicine and Diagnostics (COMManD) Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, IND.
Abstract
Background The value and use of medicinal plants, including the widespread cultivation of , have increased rapidly. , a medicinal plant native to the Mediterranean, has received attention for its potential therapeutic benefits. This study evaluates anticancer activity using human epithelial carcinoma (KB) cell lines derived from nasopharyngeal epidermoid carcinoma. The KB cell line is known for its increased sensitivity to specific chemotherapeutic agents (CA), making it a useful model in cancer research. The impact of is assessed using comprehensive analyses of cell viability and gene expression. Aim This study aims to evaluate the anti-cancer effects of on KB cell lines. Materials and methods The leaf extract was separated and used to treat KB cell lines. The cell viability of treated KB cells was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Real-time polymerase chain reaction (RT-PCR) was used to analyze the expressions of matrix metalloproteinase (MMP-9) and tumor-inducing metalloproteins (TIMP-1) messenger ribonucleic acid (mRNA) genes. The statistical analysis was performed. Results This study analyzes the anticancer properties of on KB cell lines. The results show that increasing the concentration of rosemary extract reduces cell viability in malignant cells. Furthermore, the effect on the apoptotic signaling system is demonstrated by a decrease in MMP-9 and TIMP-1 mRNA expressions, as observed by RT-PCR analysis. Conclusion Patients looking for natural anticancer treatments may benefit from biogenically prepared anticancer drugs. The current research focuses on as a potential alternative to chemically synthesized anticancer drugs.
MeSH
Cells, Genes, Pharmaceutical Preparations, Cell Survival, Polymerase Chain Reaction, Neoplasms, Kb Cells, Cell Line, Matrix Metalloproteinases, Research, Plants, Medicinal, Therapeutics, Carboplatin, Real-Time Polymerase Chain Reaction