Systematic Review Journal Article Epublish 18 citations
PubMed: 38524634 DOI: 10.3389/fendo.2024.1274376
Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis.
Front Endocrinol (Lausanne) 15:1274376
Authors: Ahmad Mohd Faizal, Marjanu Hikmah Elias, Norazilah Mat Jin, Muhammad Azrai Abu, Saiful Effendi Syafruddin, Ani Amelia Zainuddin, Nao Suzuki, Abdul Kadir Abdul Karim
Affiliations
- Department of Obstetrics & Gynecology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, Malaysia.
- Faculty of Medicine & Health Sciences, Universiti Sains Islam Malaysia, Nilai, Negeri Sembilan, Malaysia.
- Department of Obstetrics & Gynecology, Faculty of Medicine, Universiti Teknologi MARA, Jalan Hospital, Sungai Buloh, Selangor, Malaysia.
- Medical Molecular Biology Institute, National University of Malaysia, Kuala Lumpur, Malaysia.
- Department of Obstetrics & Gynecology, St Marianna School of Medicine, Kawasaki, Japan.
Abstract
The leading indicator for successful outcomes in in-vitro fertilization (IVF) is the quality of gametes in oocytes and sperm. Thus, advanced research aims to highlight the parameter in assessing these qualities - DNA fragmentation in sperm and oocyte development capacity (ODC) via evaluation of microenvironments involving its maturation process. Regarding oocytes, most evidence reveals the role of cumulus cells as non-invasive methods in assessing their development competency, mainly via gene expression evaluation. Our review aims to consolidate the evidence of GDF-9 derivatives, the HAS2, GREM1, and PTGS2 gene expression in cumulus cells used as ODC markers in relevant publications and tailored to current IVF outcomes. In addition to that, we also added the bioinformatic analysis in our review to strengthen the evidence aiming for a better understanding of the pathways and cluster of the genes of interest - HAS2, GREM1, and PTGS2 in cumulus cell level. Otherwise, the current non-invasive method can be used in exploring various causes of infertility that may affect these gene expressions at the cumulus cell level. Nevertheless, this method can also be used in assessing the ODC in various cohorts of women or as an improvement of markers following targeted tools or procedures by evaluating the advancement of these gene expressions following the targeted intervention.
MeSH
Cells, Genes, Cumulus Cells, Cyclooxygenase 2, Oocytes, Spermatozoa, Gene Expression