Journal Article Epublish 3 citations
PubMed: 38786172 DOI: 10.3390/antibiotics13050444
Essential Oil to Enhance the Effect of Imipenem against Axenic and Co-Cultures of and .
Antibiotics (Basel) 13(5)
Authors: Jorge O Fimbres-García, Marcela Flores-Sauceda, Elsa Daniela Othón-Díaz, Alfonso García-Galaz, Melvin R Tapia-Rodriguez, Brenda A Silva-Espinoza, Andres Alvarez-Armenta, J Fernando Ayala-Zavala
Affiliations
- Centro de Investigación en Alimentación y Desarrollo, A.C, Carretera Gustavo Enrique Astiazarán Rosas 46, Hermosillo 83304, Sonora, Mexico.
- Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, Col. Centro, Ciudad Obregón 85000, Sonora, Mexico.
- Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca 62250, Morelos, Mexico.
Abstract
This research focuses on assessing the synergistic effects of Mexican oregano ( essential oil or carvacrol when combined with the antibiotic imipenem, aiming to reduce the pathogenic viability and virulence of and . The study highlighted the synergistic effect of combining essential oil or carvacrol with imipenem, significantly reducing the required doses for inhibiting bacterial growth. The combination treatments drastically lowered the necessary imipenem doses, highlighting a potent enhancement in efficacy against and . For example, the minimum inhibitory concentrations (MIC) for the essential oil/imipenem combinations were notably low, at 0.03/0.000023 mg/mL for and 0.0073/0.000023 mg/mL for . Similarly, the combinations significantly inhibited biofilm formation at lower concentrations than when the components were used individually, demonstrating the strategic advantage of this approach in combating antibiotic resistance. For OXA-51, imipenem showed a relatively stable interaction during 30 ns of dynamic simulation of their interaction, indicating changes (<2 nm) in ligand positioning during this period. Carvacrol exhibited similar fluctuations to imipenem, suggesting its potential inhibition efficacy, while thymol showed significant variability, particularly at >10 ns, suggesting potential instability. With IMP-1, imipenem also displayed very stable interactions during 38 ns and demonstrated notable movement and positioning changes within the active site, indicating a more dynamic interaction. In contrast, carvacrol and thymol maintained their position within the active site only ~20 and ~15 ns, respectively. These results highlight the effectiveness of combining essential oil and carvacrol with imipenem in tackling the difficult-to-treat pathogens and .
MeSH
Oils, Volatile, Imipenem, Thymol