Journal Article Ppublish
PubMed: 38853128 DOI: 10.5653/cerm.2024.06835
The lncRNA Gm8097 is associated with hypospermatogenesis.
Clin Exp Reprod Med 51(4):314-323
Authors: Bin Lei, Luwei Ye, Zhuolin Qiu, Shoubo Zhang
Affiliations
- Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
- Department of Critical Care Medicine, The First Affiliated Hospital of Jinan University, Guangzhou, China.
- Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
- Center for Reproductive Medicine, Guangdong Armed Police Hospital, Guangzhou Medical University, Guangzhou, China.
Abstract
OBJECTIVE
To investigate whether long non-coding RNA (lncRNA) Gm8097 (LncGm8097) is associated with male infertility.
METHODS
The expression and bilogical role of LncGm8097 were investigated.
RESULTS
LncGm8097 expression was down-regulated in the testis tissues with moderate and severe hypospermatogenesis compared with those with normal spermatogenesis and mild hypospermatogenesis (p<0.05). LncGm8097 down-regulation significantly promoted apoptosis and inhibited proliferation in GC1 and GC2 cells. In addition, LncGm8097 was significantly down-regulated in mouse model of hypospermatogenesis and correlated with cell apoptosis and proliferation. LncGm8097 was located immediately upstream of PRPS2, and correlated with Bcl-2/P53/caspase 6/caspase 9 signal pathway.
CONCLUSION
LncGm8097 down-regulation correlates with hypospermatogenesis, which may offer new insights into the pathogenesis of male infertility.
MeSH
Cells, Infertility, Caspases, Oligospermia, Infertility, Male, Apoptosis, Down-Regulation, Spermatogenesis, Rna, Long Noncoding