Journal Article Review Epublish 1 citation
PubMed: 38855476 DOI: 10.1063/5.0204987
Recent advances in microfluidic-based spectroscopic approaches for pathogen detection.
Biomicrofluidics 18(3):031505
Authors: Mubashir Hussain, Xu He, Chao Wang, Yichuan Wang, Jingjing Wang, Mingyue Chen, Haiquan Kang, Na Yang, Xinye Ni, Jianqing Li, Xiuping Zhou, Bin Liu
Affiliations
- Engineering Research Center of Intelligent Theranostics Technology and Instruments, Ministry of Education, School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing 211166, China.
- Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China.
- The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou Second People's Hospital, Changzhou Medical Center, Nanjing Medical University, Changzhou 213161, China.
- Department of Laboratory Medicine, The Peoples Hospital of Rugao, Rugao Hospital Affiliated to Nantong University, Nantong 226500, China.
Abstract
Rapid identification of pathogens with higher sensitivity and specificity plays a significant role in maintaining public health, environmental monitoring, controlling food quality, and clinical diagnostics. Different methods have been widely used in food testing laboratories, quality control departments in food companies, hospitals, and clinical settings to identify pathogens. Some limitations in current pathogens detection methods are time-consuming, expensive, and laborious sample preparation, making it unsuitable for rapid detection. Microfluidics has emerged as a promising technology for biosensing applications due to its ability to precisely manipulate small volumes of fluids. Microfluidics platforms combined with spectroscopic techniques are capable of developing miniaturized devices that can detect and quantify pathogenic samples. The review focuses on the advancements in microfluidic devices integrated with spectroscopic methods for detecting bacterial microbes over the past five years. The review is based on several spectroscopic techniques, including fluorescence detection, surface-enhanced Raman scattering, and dynamic light scattering methods coupled with microfluidic platforms. The key detection principles of different approaches were discussed and summarized. Finally, the future possible directions and challenges in microfluidic-based spectroscopy for isolating and detecting pathogens using the latest innovations were also discussed.
MeSH
Environmental Monitoring, Food, Microfluidics