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Journal Article Observational Study Epublish 2 citations

PubMed: 39160465 DOI: 10.1186/s12886-024-03584-6

Unmasking of molecular players: proteomic profiling of vitreous humor in pathologic myopia.

BMC Ophthalmol 24(1):352

Authors: Yue Wen, Chi Ren, Li Zhu, Lvzhen Huang, Huijun Qi, Wenzhen Yu, Kai Wang, Mingwei Zhao, Qiong Xu

Affiliations

  • Department of Ophthalmology, Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Peking University People's Hospital, Beijing, China. College of Optometry, Peking University People's Hospital, Beijing, China. Eye Disease and Optometry Institute, Beijing, China. Peking University Health Science Center, No. 11 South Avenue of XiZhiMen, Xi Cheng District, 100044, Beijing, China.
  • Department of Ophthalmology, Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Peking University People's Hospital, Beijing, China. College of Optometry, Peking University People's Hospital, Beijing, China. Eye Disease and Optometry Institute, Beijing, China. Peking University Health Science Center, No. 11 South Avenue of XiZhiMen, Xi Cheng District, 100044, Beijing, China.
  • Department of Ophthalmology, Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Peking University People's Hospital, Beijing, China. College of Optometry, Peking University People's Hospital, Beijing, China. Eye Disease and Optometry Institute, Beijing, China. Peking University Health Science Center, No. 11 South Avenue of XiZhiMen, Xi Cheng District, 100044, Beijing, China.

Abstract

BACKGROUND

This study aimed to identify the differentially expressed proteins in the vitreous humor (VH) of eyes with and without pathologic myopia (PM), providing insights into the molecular pathogenesis.

METHODS

A cross-sectional, observational study was conducted. VH samples were collected from patients undergoing vitrectomy for idiopathic epiretinal membrane (ERM), macular hole (MH), or myopic retinoschisis (MRS). Label-free quantitative proteomic analysis identified differential protein expression, with validation using ELISA.

RESULTS

The proteomic profiling revealed significantly higher expressions of tubulin alpha 1a (TUBA1A) and eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) in PM groups (MH-PM, MRS-PM) compared to controls (MH, ERM). Conversely, xylosyltransferase 1 (XYLT1), versican core protein (VCAN), and testican-2 (SPOCK2) expressions were lower in PM. ELISA validation confirmed these findings.

CONCLUSIONS

Our study provides novel insights into the molecular mechanisms of PM. The differentially expressed proteins EEF1A1, TUBA1A, XYLT1, VCAN, and SPOCK2 may play crucial roles in chorioretinal cell apoptosis, scleral extracellular matrix (ECM) synthesis, and scleral remodeling in PM. These proteins represent potential new targets for therapeutic intervention in PM, highlighting the importance of further investigations to elucidate their functions and underlying mechanisms in disease pathogenesis.

MeSH

Cells, Eye Diseases, Myopia, Vitreous Body

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