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Publications

Journal Article Epublish 2 citations

PubMed: 39211790 DOI: 10.1021/cbmi.4c00020

Low-Frequency Coherent Raman Imaging Robust to Optical Scattering.

Chem Biomed Imaging 2(8):584-591

Authors: David R Smith, Jesse W Wilson, Siddarth Shivkumar, Hervé Rigneault, Randy A Bartels

Affiliations

  • Biomedical Imaging, Morgridge Institute for Research, Madison, Wisconsin 53715, United States.
  • Department of Electrical Engineering, Colorado State University, Fort Collins, Colorado 80523, United States.
  • School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, United States.
  • Aix Marseille University, CNRS, Centrale Med, Institut Fresnel, Marseille 13397, France. Department of Physics, University of Ottawa, Ottawa, Ontario K1N6N5, Canada.
  • Aix Marseille University, CNRS, Centrale Med, Institut Fresnel, Marseille 13397, France.
  • Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin 53715, United States.

Abstract

We demonstrate low-frequency interferometric impulsive stimulated Raman scattering (ISRS) imaging with high robustness to distortions by optical scattering. ISRS is a pump-probe coherent Raman spectroscopy that can capture Raman vibrational spectra. Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse. While this separation is simple in nonscattering specimens, such as liquids, scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum. We introduce a robust method for ISRS microscopy that works in complex scattering samples. High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.

MeSH

Pulse

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