Journal Article Ppublish 5 citations
PubMed: 39212126 DOI: 10.1002/jmri.29566
Reliability of In Vivo Creatine-Weighted Chemical Exchange Saturation Transfer (CrCEST) MRI in Calf Skeletal Muscle of Healthy Volunteers at 3 T.
J Magn Reson Imaging 61(4):1681-1692
Authors: Kasturee Chakraborty, Ritambhar Burman, Sabah Nisar, Saorla Miller, Zachary Loschinskey, Shengjie Wu, Yimei Li, Asim K Bag, Ayaz Khan, Chelsea Goodenough, Neil Wilson, Mohammad Haris, Shana E McCormack, Ravinder Reddy, Kirsten Ness, Richard Finkel, Puneet Bagga
Affiliations
- Department of Diagnostic Imaging, St Jude Children's Research Hospital, Memphis, Tennessee, USA.
- Department of Biostatistics, St Jude Children's Research Hospital, Memphis, Tennessee, USA.
- Department of Epidemiology and Cancer Control, St Jude Children's Research Hospital, Memphis, Tennessee, USA.
- Center for Advanced Metabolic Imaging in Precision Medicine, Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
- Division of Endocrinology and Diabetes, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
- Department of Pediatrics, University of Pennsylvania, Perelman School of Medicine, Philadelphia, Pennsylvania, USA.
- Department of Pediatric Medicine, St Jude Children's Research Hospital, Memphis, Tennessee, USA.
Abstract
BACKGROUND
Skeletal muscle mitochondrial oxidative phosphorylation (mtOXPHOS) is important for ATP generation and its dysfunction leads to exercise intolerance. Phosphorus magnetic resonance spectroscopy ( 31P-MRS) is a useful, noninvasive technique for mtOXPHOS assessment but has limitations. Creatine-weighted chemical exchange saturation transfer (CrCEST) MRI is a potential alternative to assess muscle bioenergetics.
PURPOSE
To evaluate the interscan repeatability, intra- and interobserver reproducibility of CrCEST during mild plantar flexion exercise.
STUDY TYPE
Retrospective.
SUBJECTS
Twenty healthy volunteers (age 37.6 ± 12.4 years, 11 females).
FIELD STRENGTH/SEQUENCE
3 T/CEST imaging using gradient echo readout.
ASSESSMENT
τCrCEST (postexercise Cr recovery time) was assessed in two scans for each participant, following mild plantar flexion exercises targeting the medial gastrocnemius (MG), lateral gastrocnemius (LG), and soleus (Sol) muscles. Three observers measured τCrCEST for interobserver reproducibility. Three readings by one observer were used to measure intraobserver reproducibility. Two scans were used for within-participant interscan repeatability.
STATISTICAL TESTS
Paired t tests, intraclass correlation coefficient (ICC), and Pearson correlation were conducted. Bland-Altman plots were used to analyze the interobserver variability. A P-value of 0.05 was considered statistically significant.
RESULTS
There was excellent intra- (ICC ) and interobserver (ICC ) reproducibility, with moderate interscan repeatability for τCrCEST in LG and MG (ICC ) and poor-to-moderate interscan repeatability in Sol (ICC ). Excellent interobserver reproducibility was confirmed by Bland-Altman plots (fixed bias P-value ).
DATA CONCLUSION
CrCEST MRI shows promise in assessing muscle bioenergetics by evaluating τCrCEST during mild plantar flexion exercise with reasonable reliability, particularly in LG and MG.
LEVEL OF EVIDENCE
4 TECHNICAL EFFICACY: Stage 1.
MeSH
Exercise, Muscles, Magnetic Resonance Imaging, Healthy Volunteers, Muscle, Skeletal, Creatine