Journal Article Ppublish 2 citations
PubMed: 39400975 DOI: 10.1111/crj.70014
Oleic Acid Inhibits SDC4 and Promotes Ferroptosis in Lung Cancer Through GPX4/ACSL4.
Clin Respir J 18(10):e70014
Authors: Jingfei Dong, Fei Qi, Huiqing Qie, Shibu Du, Li Li, Yan Zhang, Kaiyue Xu, Dehui Li, Yapei Xu
Affiliations
- Department of Clinical Laboratory, Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang, Hebei, China.
- School of Basic Medical Sciences, Chengde Medical University, Chengde, Hebei, China.
- Department of Health Care, Hebei General Hospital, Shijiazhuang, Hebei, China.
- Department of Functional Medicine, Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang, Hebei, China.
- Department of Oncology, Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang, Hebei, China.
- Gastrointestinal Endoscopy Room, Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang, Hebei, China.
Abstract
INTRODUCTION
As a common malignancy, lung cancer has a relatively poor prognosis and a low survival rate. In recent years, ferroptosis, as an emerging filed, has great promise in the potential treatment of cancer. Brucea javanica oil (BJO) is often used to treat various cancers. Oleic acid (OA) is the main ingredient of BJO. In this study, we investigated the role and molecular mechanism of OA in lung cancer treatment by promoting ferroptosis.
METHODS
In this study, A549 cells and H1299 cells were used for in vitro experiments, and a CCK-8 test, scratch test, and MTT experiment were carried out. We examined reactive oxygen species (ROS), the JC-1 probe, glutathione (GSH) expression, lipid peroxidation, SDC4 mRNA levels, and ACSL4, SLC7A11, GPX4, and SDC4 protein levels.
RESULTS
The results showed that OA could inhibit the proliferation and migration of A549 cells and H1299 cells, SDC4 was a potential therapeutic target of OA against lung cancer, and OA treatment significantly inhibited the expression of SDC4 in A549 cells and H1299 cells. OA induces ferroptosis in A549 cells and H1299 cells, decreases GSH levels, increases lipid peroxidation levels, and decreases SDC4 mRNA expression; in addition, OA upregulates ACSL4 expression and decreases SLC7A11, GPX4, and SDC4 expression.
CONCLUSION
This study confirmed that OA could inhibit SDC4 expression and promote the occurrence of ferroptosis in A549 cells and H1299 cells through the GPX4/ACSL4 pathway, providing an effective basis for the use of drugs targeting ferroptosis in lung cancer treatment.
MeSH
Cells, Lung, Respiratory Tract Diseases, Neoplasms, Ferroptosis, Lung Neoplasms, Oleic Acid, Therapeutics