Journal Article Epublish 2 citations
PubMed: 39456772 DOI: 10.3390/ijms252010988
Metagenomic Study Reveals Phage-Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases.
Int J Mol Sci 25(20)
Authors: Laura Vilà-Quintana, Esther Fort, Laura Pardo, Maria T Albiol-Quer, Maria Rosa Ortiz, Montserrat Capdevila, Anna Feliu, Anna Bahí, Marc Llirós, Adelaida García-Velasco, Mireia Morell Ginestà, Berta Laquente, Débora Pozas, Victor Moreno, Librado Jesús Garcia-Gil, Eric Jeffrey Duell, Ville Nikolai Pimenoff, Robert Carreras-Torres, Xavier Aldeguer
Affiliations
- Digestive Diseases and Microbiota Group, Girona Biomedical Research Institute (IDIBGI), 17190 Salt, Spain.
- Department of Gastroenterology, Hospital Universitari de Girona Dr. Josep Trueta, 17007 Girona, Spain.
- Hepato-Pancreato-Biliary Unit, Department of Surgery, Hospital Universitari de Girona Dr. Josep Trueta, 17007 Girona, Spain.
- Department of Pathology, Hospital Universitari de Girona Dr. Josep Trueta, 17007 Girona, Spain.
- Bioinformatics and Bioimaging (BI-SQUARED) Research Group, Biosciences Department, Faculty of Sciences, Technology and Engineering, Universitat de Vic, 08500 Vic, Spain.
- Precision Oncology Group, Girona Biomedical Research Institute (IDIBGI), 17190 Salt, Spain. Institut Català d'Oncologia (ICO), Hospital Universitari de Girona Dr. Josep Trueta, 17007 Girona, Spain.
- Hereditary Cancer Program, Institut Català d'Oncologia (ICO), Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), CIBERONC, 08908 Barcelona, Spain.
- Medical Oncology Department, Institut Català d'Oncologia (ICO), Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), 08908 Barcelona, Spain.
- Institut Català d'Oncologia (ICO), Institut de Recerca Biomedica de Bellvitge (IDIBELL), 08908 Barcelona, Spain.
- UBICS, University of Barcelona (UB), 08028 Barcelona, Spain.
- Consortium for Biomedical Research in Epidemiology and Public Health (CIBERESP), 08036 Barcelona, Spain.
- Cancer Epidemiology Research Program, Unit of Nutrition and Cancer, Institut Català d'Oncologia (ICO), Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), 08908 Barcelona, Spain.
- Department of Clinical Science, Intervention and Technology-CLINTEC, Karolinska Institutet, 14152 Stockholm, Sweden.
- Unit of Population Health, Faculty of Medicine, University of Oulu, 90220 Oulu, Finland.
Abstract
Individuals with pancreatic-related health conditions usually show lower diversity and different composition of bacterial and viral species between the gut and oral microbiomes compared to healthy individuals. We performed a thorough microbiome analysis, using deep shotgun sequencing of stool and saliva samples obtained from patients with chronic pancreatitis (CP), pancreatic ductal adenocarcinoma (PDAC), and healthy controls (HCs).We observed similar microbiota composition at the species level in both the gut and oral samples in PDAC patients compared to HCs, among which the most distinctive finding was that the abundance of oral-originated species did not differ between the oral and the gut samples. Moreover, comparing PDAC patients with HCs, was significantly more abundant in the stool samples of PDAC patients, while spp. showed higher abundance in both the oral and stool samples of PDAC patients. Finally, the most important finding was the distinctive gut phage-bacterial interactome pattern among PDAC patients. CrAssphages, particularly , showed mutual exclusion with species, while showed co-occurrence with spp., which have been shown to be capable of inducing DNA damage in human pancreatic cells ex vivo. The interactome findings warrant further mechanistic studies, as our findings may provide new insights into developing microbiota-based diagnostic and therapeutic methods for pancreatic diseases.
MeSH
Pancreatic Neoplasms, Pancreatitis, Chronic, Bacteriophages, Metagenomics, Viruses, Bacteria, Gastrointestinal Microbiome, Microbiota, Pancreatic Diseases, Oral Microbiome